Skip to main content
Download PDF

p53 in head and neck cancer: Functional consequences and environmental implications of TP53mutations

Head & Neck Oncology volume 2, Article number: 36 (2010) Cite this article

Abstract

Background

Although TP53 mutations in human tumours generally have been extensively studied, the significance of p53 in the aetiology of head and neck cancers is still incompletely characterized. In recent years, considerable interest has been focused on mutant forms of p53, the abnormal protein product of TP53 alleles with missense mutation that often accumulate in cancer cells.

Methods

We compared the nature of TP53 mutations in primary 46 head and neck squamous cell carcinomas (HNSCC) analyzed by PCR-SSCP and sequencing, immunohistochemistry, and using structural information available at IARC p53 database.

Results

Sequencing confirmed 36 TP53 mutations in 23 tumours of the 39 mutations in 26 tumours found by PCR-SSCP. Only half (17) putatively affect the function of p53 protein. Of these 8 were in the L2 domain, three affected the LSH motif and three the L3 domain. Three were in other domains. Codon 259 (GAC > GAA) which is a very rare mutation was found in 4 samples in our study. There were indications of p53 aberrations being associated with the combined effect of smoking, alcohol and work history. Patients with a negative family history of cancer had more often TP53 mutations than patients with a positive family history (71% vs. 46%).

Conclusions

Our study contributes to the knowledge of cumulative chemical exposure and p53 aberrations in head and neck cancer, an area where literature is scarce.

Introduction

Carcinomas of the head and neck are among the most common types of cancer [1] and as such represent a major health problem. Although it is difficult to distinguish the effects and risks of individual carcinogens from all other exposures, it is clear that head and neck squamous cell carcinoma (HNSCC) is epidemiologically strongly associated with alcohol consumption and exposure to tobacco smoke [2]. The probability of developing the cancer increases with the amount of tobacco and alcohol consumed [3, 4]. Associations between head and neck cancer risk and exposures to other environmental and occupational factors have also been proposed [5]. Putative occupational risk factors include nickel refining, woodworking, and exposure to textile fibres. Moreover most studies suggest that oral cancer patients have a history of diet low in fruit and vegetables [6]. In addition, human papillomavirus (HPV) infection has been associated with some HNSCC subgroups, mostly cancer in oropharynx [7, 8]. A synergistic effect between exposures is likely, because synergism has been demonstrated between smoking and radon or asbestos in lung cancer and oesophageal cancers [4, 9].

Aberrations of p53 are the most frequent molecular events in human cancers. The TP53 tumour suppressor gene in chromosome 17p13.1 encodes the p53 protein involved in many key events in the cell like regulation of cell cycle and glucose metabolism in cancer cells, DNA-repair, apoptosis, and senescence and induced by various stress signals, including DNA-damage and inflammation [10, 11]. In both mice and humans, germ line mutations in TP53 result in a strong predisposition to cancer [12]. Indeed, Gadea and co workers (2007) showed that a loss of wild-type p53 function was enough by itself to confer an increased migratory capacity to cells [13]. It has been shown that there are less TP53 mutations in the setting of HPV infection [14, 15]. The biological basis for this is provided by the fact that the HPV E6 oncoprotein specifically inactivates wild-type p53. In this way the high-risk HPV E6-mediated degradation of the p53 protein is probably an alternative pathway for a "classical" mutation to knock-out the p53 regulated pathways [15, 16]. Analysis of TP53 mutational patterns has shown its usefulness in at least two main areas [17, 18]. Firstly, knowledge of the position of mutations has helped to better understand the functions of various domains of the p53 protein and their involvement in mediating the suppressive functions that are inactivated in cancer. Secondly, it has been shown that the patterns of mutations may vary according to the nature of etiological agents implicating the use of TP53 mutation spectrum as a biomarker of environmental aetiology. Most of TP53 mutations described in the IARC TP53 mutation database affect exons 5-8, which constitute the site-specific, DNA-binding domain [19]. This region encodes for residues 130-286, also the most important region for folding and stabilization of the tertiary structure of p53 protein. Less than 2% of the mutations are found in the N- and C-terminal regulatory domains. The crystal structure of the core domain, solved in 1994, provides a template for understanding the nature of mutant p53 [20]. The structure contains a β-sandwich scaffold and a DNA-binding surface, including a loop-sheet-helix (LSH) motif and two loops (L2 and L3) tethered by a single zinc atom. Different mutations have very different consequences for the function of p53 protein. However, mostly mutation frequencies in tumours have been reported and less attention has been paid to the connection of functional state of the mutated p53 with clinical and environmental aspects of cancer. Most of the TP53 mutations in human cancers are missense mutations [17], that can either cause a loss of tumour suppressor function (LOF) or, in some cases, a gain of oncogenic function (GOF) [21, 22]. In addition to various degree of LOF, some mutant proteins inhibit the functions of the wt allele by a dominant-negative effect [19]. Recent studies have been carried out in an attempt to provide an explanation for the structural effects of most disease-related TP53 mutations [23, 24] and functional impact of TP53 mutations [25, 26].

In this study, we have analyzed p53 aberrations in primary head and neck cancer patients with information of their chemical exposures. Although the association between smoking and alcohol with head and neck cancers is well-known and quite strong [2], and it is clear that p53 aberrations in general are important in human cancers, it is not known whether p53 aberrations are associated with environmental exposures in head and neck cancers. The 46 head and neck cancers analyzed in this paper provide a significant addition the data in IARC TP53 mutation database, especially due to the known environmental exposures.

Materials and methods

Patients and tumours

The study population consisted of North Finnish patients diagnosed with a primary head and neck squamous cell carcinoma in the University Hospital of Oulu, Finland between the years 1994 and 1996. The patients were recruited to the study when entering to hospital. Details of the cases are given in Table 1. In each case, a questionnaire was filled about smoking, alcohol consumption and work place with a possibility of exposure to chemicals at work, as well as the family history. Questions were asked during the first contact with the cancer clinic by an experienced doctor or nurse. An exposure index (Table 2) was calculated using the data from the structured questionnaire on lifestyle and work history, as well as on the exposure to chemicals. The maximum rating was eight points including 0-3 points from tobacco exposure, 0-3 points from alcohol consumption and 0-2 points from possible exposure to chemicals and/or dust.

Table 1 Clinicopathological variables and p53 status in patients of head and neck carcinoma
Full size table
Table 2 The points of the exposure index
Full size table

Ethical aspect

The study design was approved by the local Research Ethics Committee of the Medical Faculty and University Hospital at the University Of Oulu, Finland (14.3.1994) and a written informed consent was obtained from all patients entering the study, after both oral and written information was given to the patients about the study. Patients were interviewed by hospital personnel (a doctor or a nurse) and the coded data was stored in a safe place by the researches. The study did not interfere with the clinical treatment of the patients.

TP53 mutation analysis strategy

Mutations in exons 5-8 of the TP53 gene were analyzed by a temperature-controlled non-radioactive single-strand conformation polymorphism (SSCP) analysis [27, 28]. A sample was judged to be positive for a TP53 mutation in SSCP only if two independent amplified PCR products contained similar shifted band patterns. The types of the TP53 mutations were further analyzed by semi-automatic sequencing.

Analysis of TP53 mutations with single-strand conformation polymorphism (SSCP)

Exons 5-8 of the TP53 gene were separately amplified by PCR using two sets of intron primers, the second set internal to the first (nested primers) [29]. Dynazyme DNA polymerase and the corresponding buffer (Finnzymes, Espoo, Finland) were used in the polymerase chain reaction (PCR) with other reagents and under the reaction conditions described previously [28]. To check for possible contamination, the first and the last reactions in each PCR series were controls with no template in the reaction. If a band appeared indicating contamination, the whole series of concurrent PCR reactions was discarded. The amplified products were purified by agarose gel electrophoresis, as described earlier [28]. In this non-radioactive SSCP method the use of two running temperatures in combination with other optimized conditions ensures 98% efficiency in mutation detection within the studied exons [27]. Pharmacia PhastSystem® semi-dry electrophoresis equipment was used for SSCP, as described earlier [28]. Two different temperatures (4°C and 20°C) were used to obtain good efficiency. Both negative and positive controls were included in each run to ensure the quality of the run. As a negative control, gel-purified, amplified normal TP53 DNA was used. The controls were confirmed to be negative by identical band patterns compared to former controls, and sequenced to be wild-type. As a positive control, DNA was amplified using artificially mutated primers [27]. The gels were stained with silver staining kit (Pharmacia Biotech, Finland) according to the instructions from the manufacture.

Sequencing of TP53gene

Once a mutation was detected by the presence of similar band shifts in SSCP from two independent PCR, the PCR amplified samples were sequenced with ABI PRISM 3100 sequencer and BigDye Terminator Sequencing Kit (Applied Biosystems, Foster City, CA).

Analysis of the effect of TP53mutation

IARC TP53 mutation database (R13, released in November 2008) was searched for the mutations found in this study [19]. The mutation validation tools were used to check the mutation data for base substitutions in the coding sequence of TP53. The following information was used: the precise description of the mutation event at the DNA and protein level, the observed (in experimental cell assays [30] or predicted (by amino-acid conservation rules or structural analysis) functional impact of the mutation, and the number of times it has been reported as a somatic or germ line mutation in the IARC TP53 database. A combination of standard structural criteria as described by Martin et al. (2002) was also used [24]. The following changes were considered to have probable functional consequences: changes in amino acids involved in hydrogen bonding (as already described by Baker and Hubbard 1984) [31], substitutions with amino acids too large to fit in the place (residue clashes), mutations to proline (cyclic side chain in proline creates a stricter backbone than other amino acids), mutations substituting glycine (able to adopt conformations sterically hindered for other amino acids), or substitutions leading to changes in direct contact with DNA or zinc binding [24].

p53 immunohistochemistry

Paraffin embedded sections (4 μm) were stained using the avidin-biotin-immunoperoxidase technique. Dewaxing (Histo-Clear®, National Diagnostic, Atlanta, GA, USA) and blocking of endogenous peroxidase and non-specific binding were carried out first. Mouse monoclonal antibody (DO-7, 1:300, Novocastra Laboratories Ltd., Newcastle upon Tyne, UK) for p53 was used as the primary antibody. The antibody recognizes both wild type and mutant forms of human p53 and the epitope is located between the amino acid residue 19 and 26. For staining the Histostain-bulk kit® (Zymed, San Francisco, CA, USA) was used. Biotinylated antimouse IgG was used as the secondary antibody and peroxidase was introduced as a streptavidin conjugate. The antibody reaction was visualised by using a fresh substrate solution containing aminoethyl carbazol (AEC-kit®, Zymed, San Francisco, CA, USA). The sections were counterstained with hematoxylin, dehydrated and mounted in glycerol-vinyl-alcohol (GVA mount®; Zymed). For the negative controls the primary antibody for p53 was replaced with mouse non-immuno IgG and each set of staining always included a separate known positive control. The slides were analysed separately by two independent observers blinded from the clinical data. The immunoreactivity in the malignant cells in each section was graded according to the number of positively staining nuclei: < 1% nuclei with a positive reaction as a negative, >1≤6% +, >6% ≤10% as ++ 11% ≤40% as +++ and > 40% as ++++.

Statistical analysis

The correlations of gender, age, primary anatomical site and exposure data were analyzed separately according to the TP53 gene mutations and p53 immunoreactivity. The statistical significance of these correlations was determined with the Fisher's exact test. Probability values of less than 0.05 were considered to be statistically significant. All statistical analyses were performed using the SPSS software system (SPSS for Windows, version 16.0, Chicago, IL).

Results

Mutations in the TP53gene in head and neck tumours

Judging by SSCP the TP53 gene was mutated in a total of 26 primary tumours (57%) in the 46 HNSCC patients with altogether 39 TP53 mutations. Sequence analysis for the exact site and nature of the genetic alterations was possible in 23 tumour samples. Eleven tumour samples (11/26, 42%) were found to harbour multiple TP53 mutations. In two cases 3 mutations were found in the same tumour and in nine cases two (Table 3). There was only a small difference in the prevalence of mutations between different tumour sites (Table 4). The majority of the mutations were missense mutations (30/36, 83%). Only one of the mutations was a nonsense mutation and two were silent. Transversions (17/31, 55%) were more frequent than transitions (14/31, 45%). The two silent mutations found in codon 170 were similar (ACG > ACA) and both were found in association with a similar codon 171 missense mutation (GAG > GAC). One of these combination mutations was found in a larynx tumour and the other in oral cavity cancer. Furthermore, codon 259 was similarly mutated in 4 samples (GAC > GAA).

Table 3 Individual mutations and p53 protein function
Full size table
Table 4 Summary of mutations
Full size table

Correlation of IHC for p53 protein with TP53mutation status

The p53 protein was analyzed in sections of the tumour samples by immunohistochemistry. In positive cases, the immunoreactive protein was prominent only in cancer cells and localized in cancer cell nuclei (Figure 1). Of the 46 primary HNSCC tumours, 24 (52%) showed a positive staining for the p53 protein. In 10 (22%) of the cases, the staining was extensive or very extensive (+++/++++), whereas 10 out of the 46 (22%) cases showed weak positivity (+) for p53 and in four cases (9%) the staining result was moderate (++). In cases where the tumour staining was very extensive or extensive for p53, 8 out of 10 cases (80%) contained also a TP53 mutation, while 17 out of 32 cases (53%) presenting with a negative or weak p53 staining contained a TP53 mutation in the tumour. The association between p53 immunohistochemical staining and the TP53 mutation status was not, however, statistically significant (P = 0.16, Fisher's exact test). There was no correlation between the type of the mutation and the positivity of p53 immunostaining.

Figure 1
figure1

Immunohistochemical staining of p53 immunoreactive protein (A, B). Nuclear immunostaining of p53 in head and neck squamous cell carcinoma. The immunostaining procedure was performed as described using an anti-p53 monoclonal antibody. A) Tumour with a very extensive (++++) immunostaining for p53 B) Tumour with a negative immunostaining for p53.

Full size image

Effect of TP53mutations on the p53 protein structure and function

According to the functional and structural domains of p53, as described in the IARC TP53 mutation database, the mutations could be classified as follows: 22% (8/36) of the mutations affect the L2 domain (between codons 164 and 194), which is needed for the correct folding and stabilization of the central part of the protein, 11% (4/36) affect the LSH (loop-sheet-helix) motif (codons 119-135 and 272-287), and 8% (3/36) affect the L3 domain (between codons 237-250), directly involved in the interaction between the protein and DNA (Table 3). According to the IARC database and based on experimental data, four of the missense mutations lead to non-functional proteins (Cys238Ser, Gly245Asp, Glu258Lys, Arg283Pro) (see Table 3). According to the predicted structure (by amino-acid conservation rules or structural analysis), several of the found mutations probably have functional impact leading to a non-functional protein (Leu130Ile, Leu130Phe, Thr155Ser, Val157Phe, Val172Ala, Arg175His, Met243Leu, Asp259Glu, Cys275Tyr). TP53 mutations leading to non-functional protein were more common in LSH and L3 motifs than in L2 motif (not statistically significant, data not shown).

Comparison of TP53gene and p53 protein alterations with patients characteristics and exposure data

Patients with a negative family history of cancer had a TP53 mutation in 71% of the cases, while patients with a positive family history (at least two cases of cancer in first-degree relatives) had a TP53 mutation in only 46% of the cases. TP53 mutations were more common (although not statistically significantly; p = 0.330) in tumours of younger patients (Table 1). All three patients under 39 years, who all had a tumour in the oral cavity, had a TP53 mutation. Otherwise TP53 mutation status was not associated with patient characteristics (Table 1). Interestingly, however, males had a p53 positive tumour more often than females (see Table 1 not statistically significant). When taking into account tobacco and alcohol consumption, the female patients had lower mean exposure (1.76 exposure index, see Table 2), than the males (3.36, p = 0.040). No statistically significant difference between the sexes was found, however, when the overall exposure was considered (p = 0.090). Another interesting finding was that p53 overexpression was more (although not statistically significantly) prevalent in laryngeal tumours than in other anatomical sites (Table 1). All tumours from patients with >45 pack years of smoking were p53 positive in ihc compared to those with 0 pack years (p = 0.021) (Table 5). No statistically significant association between p53 alterations and exposure to alcohol consumption was found. However, when all the exposures were taken into account, a positive result (p53 immunohistochemistry, TP53 mutation) seem to be more prevalent in those with a higher exposure index (Table 6). We also noticed that tobacco and alcohol exposures were significantly higher in laryngeal tumours than in oral cavity tumours (p < 0.005). No association was found between a high exposure index and the status with a non-functional protein. Unfortunately, in 37.5% of cases evaluated for functional consequences of the TP53 mutations it was not possible to [19] and that there is still minimal information about chemicals and TP53 in head and neck cancer [18] this calculate the exposure index due to partly lacking exposure data.

Table 5 Association of p53 aberrations and packyears of smoking
Full size table
Table 6 Association of p53 aberrations and exposure index
Full size table

Discussion

The most interesting finding was that we found the same mutation (Asp259Glu most probably leading to a non-functional p53 protein) in four individuals who all had chemical exposure: tobacco and alcohol, and in three cases documented work exposure to chemicals including pesticides, oil and asbestos. In the fourth case the information of work exposure was missing. Although smoking and alcohol in head and neck cancer have been linked with TP53 mutations before [32–36] work exposure has not been included in earlier papers. Furthermore, considering the fact that this mutation has been described only in five cases before in IARC database is certainly an implication to follow-up. Including our cases, out of total of nine Asp259Glu mutations four have been in larynx tumours, which may represent a preferential site for this mutation. Both of our larynx cancer patients with this mutation tumour had a high exposure index (6 and 8) and none of the patients presented a positive family history for cancer. This may justify further studies of TP53 Asp259Glu mutation as a marker of environmental exposure in larynx tumours.

The TP53 mutation frequency in this study is in line with the one reported by IARC in head and neck squamous cell carcinomas (57% in this study; 47.5% in IARC TP53 mutation database [19]. According to the IARC TP53 mutation database, mutations in head and neck cancers occur frequently in codons 238-248, which is a hotspot region. In our material this region was underrepresented with only 3/26 mutations. Among this series, 11/26 (42%) tumours contained multiple TP53 mutations. Although multiple TP53 mutations have earlier been described in the literature in HNSCCs [37–40], they are not as commonly reported as tumours with a single TP53 mutation. Our study is too small to pursue multiple mutations in connection with other parameters.

Altogether 13 tumours with TP53 mutations in our series probably harbour a non-functional protein for various reasons. For instance, zinc is essential for the function of p53, because p53 does not adopt the correct conformation in the absence of zinc [41, 42]. Thus mutations in the residues involved in the interaction with zinc, like Cys238Ser in this study, will result in a non-functional p53. According to recent data a mutation that alters the stability of the protein (structural mutants; 7 tumours in our series) is more likely to disrupt all functions of the protein, whereas a mutation within a contact residue (contact mutants; 4 cases in our series) will probably be more selective in affecting the transcriptional activity of p53 [43, 44]. Both classes of mutant p53 proteins commonly accumulate to high levels in tumour cells and are defective for wild-type in p53 functions [43]. It remains to be tested whether any relation of the functional effects of the mutations to exposure types or total exposure exists. Our series is small and the fact that we did not find such correlations does not rule them out.

In accordance with Koch and co workers (1995) our study implicated young age to associate with a higher TP53 mutation frequency in the tumours [45]. Interestingly, all the three patients under 39 had a squamous cell cancer of the oral cavity and all these tumours harboured a TP53 mutation. Petitjean et al. (2007a), on the basis of the IARC TP53 mutation database, reported that the mean age at onset in carriers of a TP53 mutation leading to a functional protein was higher than the age of patients with a non-functional protein [25]. Thus, the penetrance of a mutation may be related to its degree of loss of transcriptional activity, which is not surprising. Similar implications have been described on basis of p53 protein expression. De Paula and co-workers (2009), who evaluated 724 primary HNSCC in young (under 45 years) and older (46-92 years) patients, reported a significantly higher p53 expression (p < 0.05) and a higher incidence of oral cavity tumours in younger patients [46]. On the other hand, Regezi et al. (1999), who evaluated and compared the expression of the cell cycle proteins p53, p21, Rb and MDM2 in tongue cancer patients aged 35 and younger and those aged 75 or older, reported equivalent p53 mutant protein expression [47]. The possible association with age in different head and neck cancers still requires confirmation in larger patient materials.

Positive findings in p53 immunohistochemistry have especially earlier, been interpreted as indicating inactivation of the TP53 gene on the basis of the knowledge that the half-life of the wild-type protein is too short to permit detection, whereas the mutant protein is stable [48]. However, the TP53 gene may also harbour mutations that do not result in its stabilization, or deletions that inhibit transcription altogether. Alternatively, p53 function may be inhibited by epigenetic events, such as enhancing its degradation or by interference with proteins controlling its transcriptional activity [49]. Furthermore, p53 protein may be induced in cells by DNA-damaging chemical exposure (for reviews see [50, 51]) through posttranslational modifications [52]. In agreement with previous studies [48, 53], we found that p53 overexpression was a common event in HNSCC. In newest papers immunohistochemically positive cases associate with TP53 mutations in head and neck cancers [48, 54, 55] and our paper does not contradict this.

In the present study, we found that a positive p53 immunohistochemistry was more common among heavy smokers than among non-smokers, as reported earlier [54, 56]. However, we did not find a correlation between the amount of tobacco consumed and the frequency of TP53 mutations. Vähäkangas and co workers (2001) noticed that in lung cancer TP53 mutations occur more commonly in smokers and ex-smokers than in never-smokers [57]. Other reports pertinent to tobacco exposure, using various methods to detect TP53 mutations, have given conflicting results. Studies showing a positive association between TP53 mutation and tobacco smoke in patients with head and neck carcinoma are, however, more numerous e.g. [32–36] than studies with no association e.g. [38, 58, 59]. Unfortunately, information on alcohol consumption was lacking in eight patients in our material. Interestingly, we also found that the tumours from patients with a negative family history for cancer contained TP53 mutations more often than tumours from patients with a positive a family history. Considering the age correlation and family history data, our results may be interpreted as supporting the environmental aetiology of the TP53 mutations.

We noticed that tobacco and alcohol exposures were statistically significantly higher in laryngeal tumours than in oral cavity tumours and p53 overexpression was more prevalent in laryngeal tumours than in other anatomical sites. Recently De Paula and co-workers (2009) in a large material of over 700 patients noted a correlation between p53 immunohistochemically positive tumours and anatomical site [46]. The exposure to chemical carcinogens e.g. in smoke may not be even in different locations or the sensitivity of locations may vary. Another level of variation is the inter-individual susceptibility according to genetic factors. A small proportion of individuals exposed to potential carcinogens might develop the disease and intrinsic susceptibility to environmental exposure most probably plays a role also in head and neck cancer see e.g. [60, 61]. Furthermore, in our series females with a head and neck cancer had less exposure than males, which supports the reported higher susceptibility of women to carcinogens like cigarette smoke [62].

Conclusions

Our study shows implications of p53 aberrations being associated with the environmental exposure in head and neck cancer. Regardless of how the data are looked at, a trend for a higher frequency of p53 alterations remains among those with higher exposure. In accordance with earlier literature (see e.g. Vähäkangas 2003), our results thus justify further studies of p53 alterations as a biomarker of environmental exposure in head and neck cancers. Especially, the mutation Asp259Glu (GAC > GAA) most probably leading to a non-functional p53 protein, which was found in four tumours in this series, may justify further studies as a marker of environmental exposure in larynx tumours.

References

  1. 1.

    Ferley J, Bray F, Pisani P, Parkin DM: GLOBOCAN 2002. Cancer incidence, mortality and prevalence worldwide. 2004, IARC CancerBase No. 5. Lyon: IARC Press

    Google Scholar 

  2. 2.

    Hashibe M, Brennan P, Chuang S-C, Boccia S, Castellsague X, Chen C, Curado MP, Dal Maso L, Daudt AW, Fabianova E, Fernandez L, Wünsch-Filho V, Franceschi S, Hayes RB, Herrero R, Kelsey K, Koifman S, La Vecchia C, Lazarus P, Levi F, Lence JJ, Mates D, Matos E, Menezes A, McClean MD, Muscat J, Eluf-Neto J, Olshan AF, Purdue M, Rudnai P, Schwartz SM, Smith E, Sturgis EM, Szeszenia-Dabrowska N, Talamini R, Wei Q, Winn DM, Shangina O, Pilarska A, Zhang ZF, Ferro G, Berthiller J, Boffetta P: Interaction between tobacco and alcohol use and the risk of head and neck cancer: pooled analysis in the international head and neck epidemiology consortium. Cancer Epidemiol Biomarkers Prev. 2009, 18: 541-550. 10.1158/1055-9965.EPI-08-0347.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  3. 3.

    IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Alcohol Drinking Lyon. 1988, 44:

    Google Scholar 

  4. 4.

    IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Tobacco Smoke and Involuntary Smoking Lyon. 2004, 83:

    Google Scholar 

  5. 5.

    Siemiatycki J, Richardson L, Straif K, Latreille B, Lakhani R, Campbell S, Rousseau MC, Boffetta P: Listing occupational carcinogens. Environ Health Perspect. 2004, 112: 1447-1459. 10.1289/ehp.7047.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  6. 6.

    Edefonti V, Bravi F, La Vecchia C, Randi G, Ferraroni M, Garavello W, Franceschi S, Talamini R, Boffetta P, Decarli A: Nutrient-based dietary patterns and the risk of oral and pharyngeal cancer. Oral Oncol. 2010, 46: 343-348. 10.1016/j.oraloncology.2009.11.017.

    Article  PubMed  Google Scholar 

  7. 7.

    Chung CH, Gillison ML: Human papillomavirus in head and neck cancer: Its role in pathogenesis and clinical implications. Clin Cancer Res. 2009, 15: 6758-6762. 10.1158/1078-0432.CCR-09-0784.

    Article  CAS  PubMed  Google Scholar 

  8. 8.

    Machado J, Reis PP, Zhang T, Simpson C, Xu W, Perez-Ordonez B, Goldstein DP, Brown DH, Gilbert RW, Gullane PJ, Irish JC, Kamel-Reid S: Low prevalence of Human Papillomavirus in oral cavity carcinomas. Head & Neck Oncology. 2010, 2: 6-10.1186/1758-3284-2-6.

    Article  Google Scholar 

  9. 9.

    Nelson HH, Kelsey KT: The molecular epidemiology of asbestos and tobacco in lung cancer. Oncogene. 2002, 21: 7284-7288. 10.1038/sj.onc.1205804.

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Bensaad K, Vousden KH: p53: new roles in metabolism. Trends Cell Biol. 2007, 17: 286-291. 10.1016/j.tcb.2007.04.004.

    Article  CAS  PubMed  Google Scholar 

  11. 11.

    Hussain SP, Harris CC: p53 biological network: at the crossroads of the cellular-stress response pathway and molecular carcinogenesis. J Nippon Med Sci. 2006, 73: 54-64. 10.1272/jnms.73.54.

    Article  CAS  Google Scholar 

  12. 12.

    Lozano G, Zambetti GP: What have animal models taught us about the p53 pathway?. J Pathol. 2005, 205: 206-220. 10.1002/path.1704.

    Article  CAS  PubMed  Google Scholar 

  13. 13.

    Gadea G, de Toledo M, Anguille C, Roux P: Loss of p53 promotes RhoA-ROCK-dependent cell migration and invasion in 3 D matrices. J Cell Biol. 2007, 178: 23-30. 10.1083/jcb.200701120.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  14. 14.

    Dai M, Clifford GM, le Calvez F, Castellsague X, Snijders PJ, Pawlita M, Herrero R, Hainaut P, Franceschi S: Human papillomavirus type 16 and TP53 mutation in oral cancer: matched analysis of the IARC multicenter study. Cancer Res. 2004, 64: 468-471. 10.1158/0008-5472.CAN-03-3284.

    Article  CAS  PubMed  Google Scholar 

  15. 15.

    Gillison ML, Koch WM, Capone RB, Spafford M, Westra WH, Wu L, Zahurak ML, Daniel RW, Viglione M, Symer DE, Shah KV, Sidransky D: Evidence for a causal association between human papillomavirus and a subset of head and neck cancer. J Natl Cancer Inst. 2000, 92: 709-720. 10.1093/jnci/92.9.709.

    Article  CAS  PubMed  Google Scholar 

  16. 16.

    Tommasino M, Crawford L: Human papillomavirus E6 and E7: proteins which deregulate the cell cycle. Bioessays. 1995, 17: 509-518. 10.1002/bies.950170607.

    Article  CAS  PubMed  Google Scholar 

  17. 17.

    Hainaut P, Hollstein M: P53 and human cancer: the first ten thousand mutations. Adv Cancer Res. 2000, 77: 81-137. full_text.

    Article  CAS  PubMed  Google Scholar 

  18. 18.

    Vähäkangas K: TP53 mutations in workers exposed to occupational carcinogens. Hum Mutat. 2003, 21: 240-251. 10.1002/humu.10182.

    Article  PubMed  Google Scholar 

  19. 19.

    The IARC TP53 mutation database. [http://www-p53.iarc.fr]

  20. 20.

    Cho Y, Gorina S, Jeffrey PD, Pavletich NP: Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations. Science. 1994, 265: 346-355. 10.1126/science.8023157.

    Article  CAS  PubMed  Google Scholar 

  21. 21.

    Van Oijen MGCT, Slootweg PJ: Gain-of-function mutations in the tumor suppressor gene p53. Clin Cancer Res. 2000, 6: 2138-2145.

    CAS  PubMed  Google Scholar 

  22. 22.

    Cadwell C, Zambetti GP: The effects of wild-type p53 tumor suppressor activity and mutant p53 gain-of-function on cell growth. Gene. 2001, 277: 15-30. 10.1016/S0378-1119(01)00696-5.

    Article  CAS  PubMed  Google Scholar 

  23. 23.

    Cuff AL, Martin AC: Analysis of void volumes in proteins and application to stability of the p53 tumour suppressor protein. J Mol Biol. 2004, 344: 1199-1209. 10.1016/j.jmb.2004.10.015.

    Article  CAS  PubMed  Google Scholar 

  24. 24.

    Martin ACR, Facchiano AM, Cuff AL, Hernandez-Boussard T, Olivier M, Hainaut P, Thornton JM: Integrating mutation data and structural analysis of the TP53 tumor-suppressor protein. Hum Mutat. 2002, 19: 149-164. 10.1002/humu.10032.

    Article  CAS  PubMed  Google Scholar 

  25. 25.

    Petitjean A, Mathe E, Kato S, Ishioka C, Tavtigian SV, Hainaut P, Olivier M: Impact of mutant p53 functional properties on TP53 mutation patterns and tumor phenotype: lessons from recent developments in the IARC TP53 database. Hum Mutat. 2007, 28: 622-629. 10.1002/humu.20495. a)

    Article  CAS  PubMed  Google Scholar 

  26. 26.

    Petitjean A, Achatz MIW, Borresen-Dale AL, Hainaut P, Olivier M: TP53 mutations in human cancers: functional selection and impact on cancer prognosis and outcomes. Oncogene. 2007, 26: 2157-2165. 10.1038/sj.onc.1210302. b)

    Article  CAS  PubMed  Google Scholar 

  27. 27.

    Welsh JA, Castren K, Vähäkangas KH: Single-strand conformation polymorphism analysis to detect p53 mutations: characterization and development of controls. Clin Chem. 1997, 43: 2251-2255.

    CAS  PubMed  Google Scholar 

  28. 28.

    Hakkarainen J, Welsh JA, Vähäkangas KH: TP53 mutation detection by SSCP and sequencing. Molecular diagnosis of cancer. Methods in Molecular Medicine. Edited by: Roulston JE, Bartlett JMS. 2004, Totowa, New Jersey: Humana Press Inc, 97: 191-208. full_text. 2

    Google Scholar 

  29. 29.

    Lehman TA, Bennett WP, Metcalf RA, Welsh JA, Ecker J, Modali RV, Ullrich S, Romano JW, Appella E, Testa JR, Gerwin BE, Harris CC: p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines. Cancer Res. 1991, 51: 4090-4096.

    CAS  PubMed  Google Scholar 

  30. 30.

    Kato S, Han S-Y, Liu W, Otsuka K, Shibata H, Kanamaru R, Ishioka C: Understanding the function-structure and function-mutation relationships of p53 tumor suppressor protein by high-resolution missense mutation analysis. PNAS. 2003, 100: 8424-8429. 10.1073/pnas.1431692100.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  31. 31.

    Baker EN, Hubbard RE: Hydrogen bonding in globular proteins. Progr Biophy Mol Biol. 1984, 44: 97-179. 10.1016/0079-6107(84)90007-5.

    Article  CAS  Google Scholar 

  32. 32.

    Brennan JA, Boyle JO, Koch WM, Goodman SN, Hruban RH, Eby YJ, Couch MJ, Forastiere AA, Sidransky D: Association between cigarette smoking and mutation of the p53 gene in squamous-cell carcinoma of the head and neck. N Engl J Med. 1995, 332: 712-717. 10.1056/NEJM199503163321104.

    Article  CAS  PubMed  Google Scholar 

  33. 33.

    Liloglou T, Scholes AGM, Spandidos DA, Vaughan ED, Jones AS, Field JK: p53 mutations in squamous cell carcinoma of the head and neck predominate in a subgroup of former and present smokers with a low frequency of genetic instability. Cancer Res. 1997, 57: 4070-4074.

    CAS  PubMed  Google Scholar 

  34. 34.

    Koch WM, Lango M, Sewell D, Zahurak M, Sidransky D: Head and neck cancer in nonsmokers: a distinct clinical and molecular entity. Laryngoscope. 1999, 109: 1544-1551. 10.1097/00005537-199910000-00002.

    Article  CAS  PubMed  Google Scholar 

  35. 35.

    Ko Y, Abel A, Harth V, Bröde P, Antony C, Donat S, Fischer HP, Ortiz-Pallardo ME, Thier R, Sachinidis A, Vetter H, Bolt HM, Herberhold C, Brüning T: Association of CYP1B1 codon 432 mutant allele in head and neck squamous cell cancer is reflected by somatic mutations of p53 in tumor tissue. Cancer Res. 2001, 61: 4398-4404.

    CAS  PubMed  Google Scholar 

  36. 36.

    Ronchetti D, Neglia CB, Cesana BM, Carboni N, Neri A, Pruneri G, Pignataro L: Association between p53 gene mutations and tobacco and alcohol exposure in laryngeal squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2004, 130: 303-306. 10.1001/archotol.130.3.303.

    Article  PubMed  Google Scholar 

  37. 37.

    Cabelguenne A, Blons H, de Waziers I, Carnot F, Houllier A-M, Soussi T, Brasnu D, Beaune P, Laccourreye O, Laurent-Puig P: p53 alterations predict tumor response to neoadjuvant chemotherapy in head and neck squamous cell carcinoma: a prospective series. J Clin Oncol. 2000, 18: 1465-1473.

    CAS  PubMed  Google Scholar 

  38. 38.

    Chaves ACM, Cherubini K, Herter N, Furian R, Santos DS, Squier C, Domann FE: Charasterization of p53 gene mutation in a Brazilian population with oral squamous cell carcinomas. Int J Oncol. 2004, 24: 295-303.

    CAS  PubMed  Google Scholar 

  39. 39.

    Smardova J, Ksicova K, Binkova H, Krpensky A, Pavlova S, Rottenberg J, Koukalova H: Analysis of tumor suppressor p53 status in head and neck squamous cell carcinoma. Oncol Rep. 2004, 11: 923-929.

    CAS  PubMed  Google Scholar 

  40. 40.

    Eriksen JG, Alsner J, Steiniche T, Overgaard J: The possible role of TP53 mutation status in the treatment of squamous cell carcinomas of the head and neck (HNSCC) with radiotherapy with different overall treatment times. Radiother Oncol. 2005, 76: 135-142. 10.1016/j.radonc.2005.05.004.

    Article  CAS  PubMed  Google Scholar 

  41. 41.

    Bullock AN, Henckel J, Fersht AR: Quantitative analysis of residual folding and DNA binding in mutant p53 core domain: definition of mutant states for rescue in cancer therapy. Oncogene. 2000, 19: 1245-1256. 10.1038/sj.onc.1203434.

    Article  CAS  PubMed  Google Scholar 

  42. 42.

    Hainaut P, Mann K: Zinc binding and redox control of p53 structure and function. Antioxid Redox Signal. 2001, 3: 611-623. 10.1089/15230860152542961.

    Article  CAS  PubMed  Google Scholar 

  43. 43.

    Olive KP, Tuveson DA, Ruhe ZC, Yin B, Willis NA, Bronson RT, Crowley D, Jacks T: Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome. Cell. 2004, 119: 847-860. 10.1016/j.cell.2004.11.004.

    Article  CAS  PubMed  Google Scholar 

  44. 44.

    Yip YL, Zoete V, Scheib H, Michielin O: Structural assessment of single amino acid mutations: Application to TP53 function. Hum Mutat. 2006, 27: 926-937. 10.1002/humu.20379.

    Article  CAS  PubMed  Google Scholar 

  45. 45.

    Koch WM, Patel H, Brennan J, Boyle JO, Sidransky D: Squamous cell carcinoma of the head and neck in the elderly. Arch Otolaryngol Head Neck Surg. 1995, 121: 262-265.

    Article  CAS  PubMed  Google Scholar 

  46. 46.

    De Paula AMB, Souza LR, Farias LC, Correa GTB, Fraga CAC, Eleutério NB, Silveira AC, Santos FB, Haikal DS, Guimarães AL, Gomez RS: Analysis of 724 cases of primary head and neck squmous cell carcinoma (HNSCC) with focus on young patients and p53 immunolocalization. Oral Oncol. 2009, 45: 777-782. 10.1016/j.oraloncology.2008.11.015.

    Article  CAS  PubMed  Google Scholar 

  47. 47.

    Regezi JA, Dekker NP, McMillan A, Ramirez-Amador V, Meneses-Garcia A, Ruiz-Godoy Rivera LM, Chrysomali E, Ng IO: p53, p21. Rb, and MDM2 proteins in tongue carcinoma from patients <35 versus >75 years. Oral Oncol. 1999, 35: 379-383. 10.1016/S1368-8375(98)00126-2.

    Article  CAS  PubMed  Google Scholar 

  48. 48.

    Bosch FX, Ritter D, Enders C, Flechtenmacher C, Abel U, Dietz A, Hergenhahn M, Weidauer H: Head and neck tumor sites differ in prevalence and spectrum of p53 alterations but these have limited prognostic value. Int J Cancer. 2004, 111: 530-538. 10.1002/ijc.11698.

    Article  CAS  PubMed  Google Scholar 

  49. 49.

    Vousden KH, Lane DP: p53 in health and disease. Nat Rev Mol Cell Biol. 2007, 8: 275-283. 10.1038/nrm2147.

    Article  CAS  PubMed  Google Scholar 

  50. 50.

    Hainaut P, Vähäkangas K: p53 as a sensor of carcinogenic exposures: mechanisms of p53 protein induction and lessons from p53 gene mutations. Pathol Biol (Paris). 1997, 45: 833-844.

    CAS  Google Scholar 

  51. 51.

    Lavin MF, Guevan N: The complexity of p53 stabilization and activation. Cell Death Differ. 2006, 13: 941-950. 10.1038/sj.cdd.4401925.

    Article  CAS  PubMed  Google Scholar 

  52. 52.

    Hollstein M, Hainaut P: Massively regulated genes: the example of TP53. J Pathol. 2010, 220: 164-173.

    CAS  PubMed  Google Scholar 

  53. 53.

    Cabanillas R, Rodrigo JP, Astudillo A, Dominguez F, Suarez C, Chiara MD: P53 expression in squamous cell carcinomas of the supraglottic larynx and its lymph node metastases. Cancer. 2007, 109: 1791-1798. 10.1002/cncr.22646.

    Article  PubMed  Google Scholar 

  54. 54.

    Cruz I, Snijders PJ, Van Houten V, Vosjan M, Van der Waal I, Meijer C: Specific p53 immunostaining patterns are associated with smoking habits in patients with oral squamous cell carcinomas. J Clin Pathol. 2002, 55: 834-840. 10.1136/jcp.55.11.834.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  55. 55.

    Perrone F, Suardi S, Pastore E, Casieri P, Orsenigo M, Caramuta S, Dagrada G, Losa M, Licitra L, Bossi P, Staurengo S, Oggionni M, Locati L, Cantu G, Squadrelli M, Carbone A, Pierotti MA, Pilotti S: Molecular and cytogenetic subgroups of oropharyngeal squamous cell carcinoma. Clin Cancer Res. 2006, 12: 6643-6651. 10.1158/1078-0432.CCR-06-1759.

    Article  CAS  PubMed  Google Scholar 

  56. 56.

    Field JK, Zoumpourlis V, Spandidos DA, Jones AS: p53 expression and mutations in squamous cell carcinoma of the head and neck: expression correlates with the patients' use of tobacco and alcohol. Cancer Detect Prev. 1994, 18: 197-208.

    CAS  PubMed  Google Scholar 

  57. 57.

    Vähäkangas KH, Castre'n K, Welsh JA, Khan MA, Blömeke B, Alavanja MCR, Harris CC: p53 and K-ras mutations in lung cancers from former and never-smoking women. Cancer Res. 2001, 61: 4350-4356.

    PubMed  Google Scholar 

  58. 58.

    Obata A, Eura M, Sasaki J, Saya H, Chikamatsu K, Tada M, Iggo RD, Yumoto E: Clinical significance of p53 functional loss in squamous cell carcinoma of the oropharynx. Int J Cancer. 2000, 89: 187-193. 10.1002/(SICI)1097-0215(20000320)89:2<187::AID-IJC14>3.0.CO;2-V.

    Article  CAS  PubMed  Google Scholar 

  59. 59.

    Poeta ML, Manola J, Goldwasser MA, Forastiere A, Benoit N, Califano JA, Ridge JA, Goodwin J, Kenady D, Saunders J, Westra W, Sidransky D, Koch WM: TP53 mutations and survival in squamous-cell carcinoma of the head and neck. N Engl J Med. 2007, 357: 2552-2561. 10.1056/NEJMoa073770.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  60. 60.

    Vähäkangas K: Molecular epidemiology of human cancer risk: gene-environment interactions and p53 mutation spectrum in human lung cancer. Lung Cancer. Molecular Pathology Methods and Reviews, Methods in Molecular Medicine. Edited by: Driscoll B. 2003, Totowa, New Jersey, Humana Press, 1: 43-59.

    Google Scholar 

  61. 61.

    Llewellyn CD, Linklater K, Bell J, Johnson NW, Warnakulasuriya KA: Squamous cell carcinoma of the oral cavity in patients aged 45 and under: a descriptive analysis of 116 cases diagnosed in the South East of England from 1990-1997. Oral Oncol. 2003, 39: 106-114. 10.1016/S1368-8375(02)00026-X.

    Article  CAS  PubMed  Google Scholar 

  62. 62.

    Toyooka S, Tsuda T, Gazdar AF: The TP53 gene, tobacco exposure, and lung cancer. Hum Mutat. 2003, 21: 229-239. 10.1002/humu.10177.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank Ms. Virpi Koponen, Ms. Kaisu Järvenpää, Ms. Anne Bisi, Ms. Tuulikki Kärnä and Ms. Kaisa Penttilä for skillful technical assistance. Dr. Curtis C Harris and Ms. Judith A Welsh have kindly provided the PCR primers for p53. The work has been financially supported by the Finnish Academy, Finnish Cancer Societies, Cancer Society of Northern Finland, and K. Albin Johansson Foundation.

Author information

Affiliations

  1. Institute of Clinical Medicine, Department of Oncology and Radiotherapy, University of Oulu, Oulu University Hospital, Oulu, Finland

    Jenni K Peltonen, Henni M Helppi & Taina Turpeenniemi-Hujanen

  2. Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Oulu, Oulu, Finland

    Jenni K Peltonen

  3. Institute of Diagnostic, Department of Pathology, University of Oulu, Oulu University Hospital, Oulu, Finland

    Paavo Pääkkö

  4. Faculty of Health Sciences, University of Eastern Finland, POB 1627, FI-70211, Kuopio, Finland

    Kirsi H Vähäkangas

Authors
  1. Jenni K Peltonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Henni M Helppi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paavo Pääkkö
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Taina Turpeenniemi-Hujanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kirsi H Vähäkangas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jenni K Peltonen.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

JKP carried out mutation analysis, analyzed the mutation data and wrote the paper with KHV. HMH carried out immunohistochemical analysis and statistical analysis, wrote relevant methods parts and commented the manuscript. PP provided pathology expertise and commented the manuscript, T-TH took part in designing the study, was responsible for identifying and interviewing the patients and commented the manuscript, KHV designed and supervised the study, took part in the analysis of data, wrote the manuscript with JKP and edited the manuscript. All authors approved the final manuscript.

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Peltonen, J.K., Helppi, H.M., Pääkkö, P. et al. p53 in head and neck cancer: Functional consequences and environmental implications of TP53mutations. Head Neck Oncol 2, 36 (2010). https://doi.org/10.1186/1758-3284-2-36

Download citation

Keywords

  • Neck Cancer
  • TP53 Mutation
  • TP53 Gene
  • Negative Family History
  • Oral Cavity Cancer

Head & Neck Oncology

ISSN: 1758-3284