- Open Access
An evaluation of the preoperative hemoglobin level as a prognostic factor for oral squamous cell carcinoma
© Cordella et al; licensee BioMed Central Ltd. 2011
- Received: 12 July 2011
- Accepted: 15 August 2011
- Published: 15 August 2011
Hypoxia seems to be an influencing factor for oral squamous cell carcinomas (SCC), and several immunohistochemical markers have been discussed in this regard. The aim of the present study was to evaluate preoperative hemoglobin levels as a prognostic factor for oral SCC.
Materials and methods
The files of 287 patients who had been treated for oral SCC between 1999 and 2008 were studied retrospectively. Hemoglobin levels between 1 and 5 days prior to surgical treatment were compared to Tumor (T)- and Nodal (N)- status, local recurrence, and lymph node metastases rate. The minimum follow-up period was 12 months.
From a total of 287 patients with oral SCC, 205 (71.4%) were in the normal hemoglobin (Hb) group (female Hb≥12.0 g/dl; male Hb≥13.0 g/dl), 53 (18.5%) in the mild anemia (female Hb = 11.0-11.9 g/dl; male Hb = 11.0-12.9 g/dl), and 29 (10.1%) in the severe anemia group (female & male Hb<11.0 g/dl). Anemia was significant for the development of lymph node metastasis (p = 0.005) as well as for local recurrence (p = 0.001). No significant correlation was found to the initial T status (p = 0.183).
Our data suggests that an Hb of below 11 g/dl contributes to and is an indicator for a poor prognosis. Consequently, pre-treatment Hb corrections may significantly improve outcome, but further investigations, including blood transfusion/application of erythropoietin due to tumor anemia, independent of intraoperative blood-loss are necessary to ascertain their role in an improved survival.
- Human Papilloma Virus
- Oral Squamous Cell Carcinoma
- Oral Squamous Cell Carcinoma
- Oral Squamous Cell Carcinoma Patient
- Tumor Oxygenation
The estimated number of newly diagnosed cancers of the oral cavity and pharynx is 97,800 per year in Europe; the estimated number of deaths due to these carcinomas is 40,100 per year . Despite improved diagnostic tools, chemotherapy, radiotherapy, and improved surgical techniques, the 5-year survival rate for head and neck cancer seems to be unchanged over the last two decades.
Clinical features and progression can differ greatly: some tumors develop no metastases, while others infiltrate at a very early stage and develop lymphangiomatosis or perineural invasion; such complications are not limited to well known risk factors such as smoking, alcohol consumption, poor mouth hygiene, or human papilloma virus (HPV). But the reason why the outcomes of some tumors are so much worse than those of others has still not been discovered. One of the most important prognostic factors for lymph node metastasis in head and neck cancer is tumor infiltration depth, but the reason for different histological growth patterns and lymphogenic metastases is still unknown.
Hypoxia seems to be an influencing factor for oral squamous cell carcinomas (SCC), and it is well known that the radiation doses needed to kill hypoxic cells are approximately 2-3 times higher than those needed to destroy well-oxygenated cells[3, 4]. Most studies for head and neck SCC, however, deal with the effect of anemia as it relates to the outcome of radiotherapy[5, 6]. Therefore, the question arises whether anemia also influences the outcome of surgical treatment. We have come across no studies which have dealt with this subject.
A total of 287 patients who were treated for oral SCC over a 10-year period between 1999 and 2008 at a single center (Clinic for Craniomaxillofacial and Oral Surgery, University Hospital Zurich) were evaluated retrospectively. All hemoglobin levels between 1 and 5 days prior to surgical treatment, Tumor (T)- and Nodal (N)-statuses, local recurrence rates, and lymph node metastases were taken into consideration. The minimum follow-up time was 12 months. Due to inadequate information and/or a follow-up time of less than 12 months, 82 patients were excluded.
Using the normal hemoglobin (Hb) values defined by the World Health Organization (WHO), the patients were divided into a three groups: normal (female Hb≥12.0 g/dl; male Hb≥13.0 g/dl), mild anemia (female Hb = 11.0-11.9 g/dl; male Hb = 11.0-12.9 g/dl), and severe anemia (female & male Hb<11.0 g/dl), according to Becker et al. 
All data were primarily obtained for medical purpose under informed consent of the patients. The study design fulfills the criteria of paragraphs 4a and b according to the guidelines (version 21.5.2010.2010) of the cantonal ethics committee of Zurich and therefore is exempted from institutional review board approval. The study design thereby fulfills the guidelines of the Declaration of Helsinki about Ethical Principles for Medical Research Involving Human Subjects.
For statistical analysis, SPPS 18 for Mac was used. P value < 0.05 was considered to be statistically significant. Kaplan-Meier analysis with log-rank testing was used for analysis.
It is widely accepted that anemia causes radiation resistance because the Hb level presumably mediates tumor response to radiation through the delivery of oxygen to the tumor. There is increasing evidence that low Hb levels are indeed associated with poor tumor oxygenation, and increasing Hb concentrations are correlated with higher pO2 levels and lower hypoxic tissue fractions. Therefore, several authors advocate blood transfusions before radiotherapy for anemic patients[8–10].
In the current study, low Hb levels were found to be associated with the development of lymph node metastasis (p = 0.005). Concerning the development of local recurrence, anemia is also a prognostic factor (p = 0.001), but, surprisingly, not the initial T status (p = 0.183). These data suggest that a low Hb concentration contributes to poor prognosis; these findings were supported by the results of several previous studies[11, 12].
One reason could be that hypoxia is induced via Hypoxia-inducible Factor-1α (HIF-1α) and Vascular endothelial growth factor (VEGF), leading to a loss of p53.
If a low Hb concentration is a predictor of decreased local control, consequently, Hb corrections may significantly improve tumor oxygenation.
On the other hand, out of 29 patients in the severe anemia group, 17 had T4 tumors; therefore, large/infiltrative tumors might have led to anemia. However our data did not reveal T-status influence on local recurrence or lymph node metastases. Another reason could be the advanced stage of the disease. In the present study, 65.5% of patients with severe anemia had lymph node metastases, but, in the normal group, only 28.1% had positive lymph nodes.
Studies regarding blood transfusion in head and neck cancer patients
(141 with transfusion)
Recurrence, survival, infection
(46 with transfusion)
Head and neck
(113 with transfusion)
(99 with tranfusion)
Head and neck
P < 0.009
(51 with transfusion)
Head and neck
(132 with transfusion)
Head and neck
(152 with transfusion)
Head and neck
P < 0.05
(38 with transfusion)
P < 0.05
(25 with transfusion)
Head and neck
(48% with transfusion; 24% without transfusion)
(86 with transfusion)
165 (60 with transfusion)
Head and neck
P < 0.04
(64 with transfusion)
P < 0.01
(437 with transfusion)
≥3 units influence
For patients with colorectal, lung, or breast carcinoma, an immunosuppressive effect of blood transfusions leading to increased local recurrence has been discussed, but is doubted by some authors[18, 19], and it has not been proven so far for head and neck cancer. There is also some laboratory evidence for an association between blood transfusion and tumor growth.
One hypothesized mechanism for a worsened prognosis in tumor patients undergoing blood transfusion is the presence of biologically active growth factors in stored blood products. These factors seem to leach from red blood cells [21–23]
The strength of this study lays in the high number of patients with a minimum follow-up time (12 months) compared to smaller numbers of patients with head and neck cancer written about in the literature.
The limitation of the study is its retrospective nature. Conversely, the analysis was restricted to those variables that could be retrieved reliably for every patient from our database. Therefore, the number of blood transfusions and the amount of intraoperative blood-loss were excluded. In addition, other risk factors such as smoking, alcohol use, and poor mouth hygiene were not included because that information was not known. The number of patients with T3 status in this study is low and not consistent with T-status distribution of SCC found in many other studies and is not the distribution one would expect. However, this fact should not influence the results.
The idea of advocating preoperative transfusion or erythropoietin administration prior to surgery has very important economic as well as physiologic consequences and, given the grade of evidence, we consider this idea only with caution. Further investigations are needed, including examination of blood transfusion/application of erythropoietin due to tumor anemia in a prospective setting with multivariate analysis to rule out dependency with other more important factors. The influence of all negative side effects possibly coming with the transfusion of blood products also needs to be addressed.
Also there are several reasons why patients with head and neck tumors may have low preoperative hemoglobin, such as nutritional deficiency, difficulties involving oral intake, and/or social deprivation. Anemia could also be a marker for other risk factors, such as p53 mutation, loss of heterozygosity (LOH), HPV, etc. These factors should also be considered in future prospective studies.
In sum, the results of this study were not expected and no satisfying explanation is obvious. However, if it is found that Hb is not independent, it might still be an important and easily obtained marker for underlying conditions the cause for poor prognosis.
Our data suggest that a low Hb level contributes to poor prognosis in oral SCC patients. Consequently, Hb corrections may significantly improve outcomes, but further investigations are necessary to clear the role of Hb in outcome prediction for oral SCC.
- Boyle P, Ferlay J: Cancer incidence and mortality in Europe, 2004. Ann Oncol. 2005, 16: 481-488. 10.1093/annonc/mdi098.View ArticlePubMedGoogle Scholar
- Woolgar JA, Hall GL: Determinants of outcome following surgery for oral squamous cell carcinoma. Future Oncol. 2009, 5: 51-61. 10.2217/147966184.108.40.206.View ArticlePubMedGoogle Scholar
- Gray LH, Conger AD, Ebert M, Hornsey S, Scott OC: The concentration of oxygen dissolved in tissues at the time of irradiation as a factor in radiotherapy. Br J Radiol. 1953, 26: 638-648. 10.1259/0007-1285-26-312-638.View ArticlePubMedGoogle Scholar
- Evans NTS, Naylor PFD: The effect of oxygen breathing and radiotherapy upon the tissue oxygen tension of some human tumours. Br J Radiol. 1963, 36: 418-425. 10.1259/0007-1285-36-426-418.View ArticleGoogle Scholar
- Becker A, Stadler P, Lavey RS, Hansgen G, Kuhnt T, Lautenschlager C, Feldmann HJ, Molls M, Dunst J: Severe anemia is associated with poor tumor oxygenation in head and neck squamous cell carcinomas. Int J Radiat Oncol Biol Phys. 2000, 46: 459-466. 10.1016/S0360-3016(99)00384-3.View ArticlePubMedGoogle Scholar
- Brizel DM, Dodge RK, Clough RW, Dewhirst MW: Oxygenation of head and neck cancer: changes during radiotherapy and impact on treatment outcome. Radiother Oncol. 1999, 53: 113-117. 10.1016/S0167-8140(99)00102-4.View ArticlePubMedGoogle Scholar
- Vaupel P, Mayer A, Hockel M: Impact of hemoglobin levels on tumor oxygenation: the higher, the better?. Strahlenther Onkol. 2006, 182: 63-71. 10.1007/s00066-006-1543-7.View ArticlePubMedGoogle Scholar
- Warde P, O'Sullivan B, Bristow RG, Panzarella T, Keane TJ, Gullane PJ, Witterick IP, Payne D, Liu FF, McLean M, et al: T1/T2 glottic cancer managed by external beam radiotherapy: the influence of pretreatment hemoglobin on local control. Int J Radiat Oncol Biol Phys. 1998, 41: 347-353. 10.1016/S0360-3016(98)00062-5.View ArticlePubMedGoogle Scholar
- Kumar P: Tumor hypoxia and anemia: impact on the efficacy of radiation therapy. Semin Hematol. 2000, 37: 4-8.View ArticlePubMedGoogle Scholar
- Kumar P: Impact of anemia in patients with head and neck cancer. Oncologist. 2000, 5 (Suppl 2): 13-18.View ArticleGoogle Scholar
- Hefler L, Mayerhofer K, Leibman B, Obermair A, Reinthaller A, Kainz C, Tempfer C: Tumor anemia and thrombocytosis in patients with vulvar cancer. Tumour Biol. 2000, 21: 309-314. 10.1159/000030136.View ArticlePubMedGoogle Scholar
- Qiu MZ, Yuan ZY, Luo HY, Ruan DY, Wang ZQ, Wang FH, Li YH, Xu RH: Impact of pretreatment hematologic profile on survival of colorectal cancer patients. Tumour Biol. 2010Google Scholar
- Blancher C, Harris AL: The molecular basis of the hypoxia response pathway: tumour hypoxia as a therapy target. Cancer Metastasis Rev. 1998, 17: 187-194. 10.1023/A:1006002419244.View ArticlePubMedGoogle Scholar
- Sturgis EM, Congdon DJ, Mather FJ, Miller RH: Perioperative transfusion, postoperative infection, and recurrence of head and neck cancer. South Med J. 1997, 90: 1217-1224. 10.1097/00007611-199712000-00011.View ArticlePubMedGoogle Scholar
- Taniguchi Y, Okura M: Prognostic significance of perioperative blood transfusion in oral cavity squamous cell carcinoma. Head Neck. 2003, 25: 931-936. 10.1002/hed.10313.View ArticlePubMedGoogle Scholar
- Fenner M, Vairaktaris E, Nkenke E, Weisbach V, Neukam FW, Radespiel-Troger M: Prognostic impact of blood transfusion in patients undergoing primary surgery and free-flap reconstruction for oral squamous cell carcinoma. Cancer. 2009, 115: 1481-1488. 10.1002/cncr.24132.View ArticlePubMedGoogle Scholar
- Schuller DE, Scott C, Wilson KM, Freer R, al-Sarraf M, Jacobs J, Ahmad K, Casiano R, Laramore G: The effect of perioperative blood transfusion on survival in head and neck cancer. Arch Otolaryngol Head Neck Surg. 1994, 120: 711-716.View ArticlePubMedGoogle Scholar
- Akyol AM, Galloway DJ, George WD: Perioperative blood transfusion does not promote recurrence and death after mastectomy for breast cancer. Br J Surg. 1992, 79: 470-View ArticlePubMedGoogle Scholar
- Donohue JH, Williams S, Cha S, Windschitl HE, Witzig TE, Nelson H, Fitzgibbons RJ, Wieand HS, Moertel CG: Perioperative blood transfusions do not affect disease recurrence of patients undergoing curative resection of colorectal carcinoma: a Mayo/North Central Cancer Treatment Group study. J Clin Oncol. 1995, 13: 1671-1678.PubMedGoogle Scholar
- Francis DM, Shenton BK: Blood transfusion and tumour growth: evidence from laboratory animals. Lancet. 1981, 2: 871-View ArticlePubMedGoogle Scholar
- Upile T, Jerjes W, Mahil J, Sudhoff H, Hopper C, Wright A: Blood product transfusion and cancer prognosis. Clin Adv Hematol Oncol. 2009, 7: 656-661.PubMedGoogle Scholar
- Upile T, Jerjes W, Mahil J, Upile N, Sudhoff H, Wright A, Hopper C: An explanation for the worsened prognosis in some cancer patients of perioperative transfusion: the time-dependent release of biologically active growth factors from stored blood products. European archives of oto-rhino-laryngology: official journal of the European Federation of Oto-Rhino-Laryngological Societies. 2011Google Scholar
- Upile T, Jerjes W, Sandison A, Singh S, Rhys-Evans P, Sudhoff H, Hopper C: The direct effects of stored blood products may worsen prognosis of cancer patients; shall we transfuse or not? An explanation of the adverse oncological consequences of blood product transfusion with a testable hypothesis driven experimental research protocol. Medical hypotheses. 2008, 71: 489-492. 10.1016/j.mehy.2008.04.027.View ArticlePubMedGoogle Scholar
- Bock M, Grevers G, Koblitz M, Heim MU, Mempel W: Influence of blood transfusion on recurrence, survival and postoperative infections of laryngeal cancer. Acta Otolaryngol. 1990, 110: 155-160. 10.3109/00016489009122531.View ArticlePubMedGoogle Scholar
- Jones KR, Weissler MC: Blood transfusion and other risk factors for recurrence of cancer of the head and neck. Arch Otolaryngol Head Neck Surg. 1990, 116: 304-309.View ArticlePubMedGoogle Scholar
- Ell SR, Stell PM: Blood transfusion and survival after laryngectomy for laryngeal carcinoma. J Laryngol Otol. 1991, 105: 293-294.View ArticlePubMedGoogle Scholar
- Woolley AL, Hogikyan ND, Gates GA, Haughey BH, Schechtman KB, Goldenberg JL: Effect of blood transfusion on recurrence of head and neck carcinoma. Retrospective review and meta-analysis. Ann Otol Rhinol Laryngol. 1992, 101: 724-730.View ArticlePubMedGoogle Scholar
- von Doersten P, Cruz RM, Selby JV, Hilsinger RL: Transfusion, recurrence, and infection in head and neck cancer surgery. Otolaryngol Head Neck Surg. 1992, 106: 60-67.PubMedGoogle Scholar
- Barra S, Barzan L, Maione A, Cadelano A, Pin M, Franceschi S, Comoretto R: Blood transfusion and other prognostic variables in the survival of patients with cancer of the head and neck. Laryngoscope. 1994, 104: 95-98.View ArticlePubMedGoogle Scholar
- Alun-Jones T, Clarke PJ, Morrissey S, Hill J: Blood transfusion and laryngeal cancer. Clin Otolaryngol Allied Sci. 1991, 16: 240-244. 10.1111/j.1365-2273.1991.tb00921.x.View ArticlePubMedGoogle Scholar
- Leon X, Quer M, Luz Maestre M, Burgues J, Muniz E, Madoz P: Blood transfusions in laryngeal cancer: effect on prognosis. Head Neck. 1996, 18: 218-224. 10.1002/(SICI)1097-0347(199605/06)18:3<218::AID-HED2>3.0.CO;2-2.View ArticlePubMedGoogle Scholar
- Moir MS, Samy RN, Hanasono MM, Terris DJ: Autologous and heterologous blood transfusion in head and neck cancer surgery. Arch Otolaryngol Head Neck Surg. 1999, 125: 864-868.View ArticlePubMedGoogle Scholar
- Szakmany T, Dodd M, Dempsey GA, Lowe D, Brown JS, Vaughan ED, Rogers SN: The influence of allogenic blood transfusion in patients having free-flap primary surgery for oral and oropharyngeal squamous cell carcinoma. Br J Cancer. 2006, 94: 647-653. 10.1038/sj.bjc.6603013.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.