1-Raman spectroscopy can be applied to a wide range of sample morphologies, including single crystals, films, fibres', suspensions, aggregates, or precipitates.

2-Samples require minimal preparation-no need for fixation or staining.

3-Can provide a high degree of information which not easy to obtain by other methods.

4-In comparison with other diagnostic techniques, Raman spectroscopy require small amount of sample.

5-Non destructive, non-invasive method for medical applications.

6-Raman spectra can be acquired quickly.

7-Raman spectroscopy is ideal for studying biological matter.

1-A significant problem associated with Raman applications arises from inherently weak signal produced by the Raman Effect.

2-Biomedical samples are extremely intricate systems which reflect complex Raman spectra. Raman band due to biological constitutes are generally overlapped, making it difficult to identify individual components correctly.

3-Due to the minimal sample preparation encountered in the clinical environment, biomedical sample samples usually produce a strong fluorescent background which may completely obscure the true Raman signals.

Raman spectroscopy is a useful diagnostic method because it is non-invasive and non-destructive. It can either be viewed as a complimentary method to biopsy or an alternative method in some instances. The technique is fast and can be used in situ. It is a technique that is comparable to fluorescence and is not only used for diagnosis of oral cancer, but potentially has many uses in the medical field.