Figure 1From: Knockdown of aberrantly expressed nuclear localized decorin attenuates tumour angiogenesis related mediators in oral cancer progression model in vitroEffect of nuclear decorin silencing on cytokines/chemokines expression in decorin silenced DOK and SCC-25 cells. DOK and SCC-25 cells were stably transfected with decorin-shRNA (DCN-shRNA), or scrambled sequence-shRNA (Ctrl-shRNA) or no transfection control (WT). RNA was extracted and cDNA was subjected to multiplex RT-PCR to detect multiple cytokine transcripts. MPCR products were fractionated electrophoretically on a 2% agarose gel containing 0.5 mg/ml ethidium bromide, visualized under UV light and photographed. The results shown are representative of three independent experiments.Back to article page