Figure 5

Migration and invasion suppression rescued by addition of IL8 in decorin silenced cell lines. Decorin silenced DOK and SCC-25 cells were cultured with 2.5 ng/ml of recombinant human IL-8 (R&D Systems, Minneapolis, MN) or conditioned medium (CM) from respective wild type cell lines for 24 h prior to migration and invasion assay. A, Cell motility through uncoated filters (migration) was measured 22 h after plating. The migrating cells were fixed, stained, and photographed as described in materials and methods. A, Migrated cells in each one of the five fields of duplicate filters were counted, numbers represent mean ± SD of three experiments. B, Cells that invaded across the Matrigel™ layer were fixed, stained, and photographed. Migrated and invaded cells in five fields of duplicate filters were counted and % invasion was calculated as described in materials and methods. Numbers represent mean ± SD of three individual experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to respective controls.