Figure 2From: A role for aberrantly expressed nuclear localized decorin in migration and invasion of dysplastic and malignant oral epithelial cellsDecorin silencing does not affect DOK or SCC-25 cell growth/proliferation. WT, control and decorin silenced DOK and SCC-25 cells were cultured for 24 h. During the last hour of culture, 20 μl of CellTiter 96® Aqueous One Solution Reagent containing a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES) was added to the media (100 μl per well), and color changes were recorded by absorbance at 490 nm. Data are presented as mean ± SE of three replicates of one representative experiment of three.Back to article page