Head & Neck Oncology

Table 3 Oncogene and cytokine analysis of HNSCC cell lines by qRT-PCR.

From: Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1

	USC-HN1	FaDu
Gene	Average Fold Δ	Average Fold Δ	t-test
p53	0.2111	0.3671	0.5377
Rb	1.1222	0.5076	0.2022
*c-myc*	0.9730	0.5848	0.0025
*c-Kit*	0.0006	0.0002	0.0286
VEGFa	0.4272	0.1406	0.0026
VEGFc	0.2402	0.1450	0.7093
COX2	0.0385	0.0064	0.4963
TGF-B1	1.1887	0.1312	0.0205
TGF-B2	0.0261	0.0172	0.2514
IL-1B	0.2091	29.4400	0.0064
IL-4	0.3472	0.0767	0.0660
IL-6	0.0661	0.0905	0.1403
IL-8	0.1866	0.1293	0.1389
IL-10	0.0204	0.0078	0.3217

RNA was extracted from USC-HN1 and FaDu cell lines, treated with DNase, and analyzed against Universal Human Reference RNA. Gene amplification was normalized to GAPDH. Analysis of oncogenes and cytokines depict an overall similar profile for both USC-HN1 and FaDu. C-myc, c-Kit, VEGFa, and TGF-B1 are significantly upregulated in USC-HN1 compared to FaDu; IL-1B was significantly down-regulated.

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