Head & Neck Oncology

Table 2 Analysis of USC-HN1 cytokines and surface markers by FACS.

From: Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1

	% Positive		MFI
Target	Isotype Control	Antibody	Isotype Control	Antibody
FABP5	0.01	97.49	31.80	1310.47 ***
E-cadherin	0.01	49.74	6.87	203.69 ***
CD24	0.01	7.87	6.87	90.52 ***
EGFR	4.50	10.71	37.70	198.78 **
c-Kit	0.01	0.26	6.55	14.01 **
CD74	0.01	0.15	6.87	9.16 **
IL-13Rα	5.47	2.63	8.89	7.89 *
CD44v6	0.06	0.04	13.27	9.38 *
CD133	7.57	3.02	38.84	20.67 *

*** > 1000% Control MFI
** 100-1000% Control MFI
* < 100% Control MFI
USC-HN1 cells in culture were stained for HNSCC-related cytokines and surface markers (left column) using monoclonal antibodies and analyzed by flow cytometry. Samples were run in duplicate. Percent of positive staining cells (middle columns) and mean fluorescence intensity (right columns) is shown for each antibody target and its isotype control.

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