Table 1 Cytokine and Oncogene Primers for qRT-PCR
Target | Forward Primer | Reverse Primer |
|---|---|---|
p53 | 5' - GCTCGACGCTAGGATCTGAC - 5' | 5' - CAGGTAGCTGCTGGGCTC - 3' |
Rb | 5' - GTTGGTCCTTCTCGGTCCTT - 3' | 5' - CAAAGCAGAAGGCAACTTGA - 3' |
c-myc | 5' - CTCCTCCTCGTCGCAGTAGA - 3' | 5' - GCTGCTTAGACGCTGGATTT - 3' |
c-Kit | 5' - GCCCACGCGGACTATTAAGT - 3' | 5' - CTGGGATTTTCTCTGCGTTC - 3' |
VEGF-A | 5' - CACACAGGATGGCTTGAAGA - 3' | 5' - AGGGCAGAATCATCACGAAG - 3' |
VEGF-C | 5' - CTCCAGATCTTTGCTTGCAT - 3' | 5' - CTGTGGCGTGTTCTCTGCT - 3' |
COX2 | 5' - TTCAAATGAGATTGTGGGAAAATTGCT - 3' | 5' - AGATCATCTCTGCCTGAGTATCTT - 3' |
TGFβ-1 | 5' - GCAGAAGTTGGCATGGTAGC - 3' | 5' - CCCTGGACACCAACTATTGC - 3' |
TGFβ-2 | 5' - CTCCATTGCTGAGACGTCAA - 3' | 5' - CGACGAAGAGTACTACGCCA - 3' |
IL-1β | 5' - GGAGATTCGTAGCTGGATGC - 3' | 5' - GAGCTCGCCAGTGAAATGAT - 3' |
IL-4 | 5' - AGCGAGTGTCCTTCTCATGG - 3' | 5' - CAGCCTCACAGAGCAGAAGA - 3' |
IL-6 | 5' - CATTTGTGGTTGGGTCAGG - 3' | 5' - AGTGAGGAACAAGCCAGAGC - 3' |
IL-8 | 5' - AGCACTCCTTGGCAAAACTG - 3' | 5' - CAAGAGCCAGGAAGAAACCA - 3' |
IL-10 | 5' - GCCACCCTGATGTCTCAGTT - 3' | 5' - GTGGAGCAGGTGAAGAATGC - 3' |
GAPDH | 5' - CTCTGCTCCTCCTGTTCGAC - 3' | 5' - TTAAAAGCAGCCCTGGTGAC - 3' |