|
Type of primer
|
Nucleotide sequence (5-3)
|
|---|
|
DSE-UF
|
GTGGAGGTGATGTTGGAGAGAAT
|
|
DSE-MF
|
AGGCGACGTCGGAGAGAAC
|
|
DSE-UR
|
AAACTACCCACCCAAAACTCCCA
|
|
DSE-MR
|
TACCCGCCCGAAACTCCCG
|
|
D4ST-UF
|
GGGTGGAGAGTGGTTGGGT
|
|
D4ST-MF
|
GTGGAGAGCGGTCGGGC
|
|
D4ST-UR
|
AATAACCAACTCCCAAACTACAACA
|
|
D4ST-MR
|
CCGACTCCCGAACTACGACG
|
|
C4ST-UF
|
TTAGATGGTGGTTGAGTTT
|
|
C4ST-MF
|
TTAGACGGCGGTCGAGTTC
|
|
C4ST-UR
|
AAAATATAATCACATCCAACTATTAACCA
|
|
C4ST-MR
|
ATCGCGTCCAACTATTAACCG
|
- The longer segments of the unmethylated primers are in boldface type and differences between methylated/modified and ummethylated/modified sequence are underlined underlined
