Figure 2

Bioluminescence imaging of mice orthotopically transplanted with luciferase-transduced cells. Bioluminescence imaging of mice orthotopically implanted with luciferase-transduced OSC-19 LN2-Luc cells. Mice were imaged using the IVIS 200 imaging system (Xenogen Corporation, Berkeley, CA). In brief, mice were first injected intraperitoneally with 40 mg/kg luciferin (Xenogen Corporation) and were anesthetized with 2% isoflurane (Abbott Laboratories, Chicago, IL). On day 7, mice had no cervical lymph node metastases. By day 12, mice showed a progression of tumor spread from the tongue to the cervical lymph nodes. The primary tumor was removed surgically on day 13 to allow for increased animal survival and to allow time for the lymph node metastases to grow and further develop. On day 82, increased intensity with cervical lymph node metastasis was observed.