Expression of Glut-1, HIF-1α, PI3K and p-Akt in a case of ceruminous adenoma
© Shen et al.; licensee BioMed Central Ltd. 2012
Received: 1 April 2012
Accepted: 2 May 2012
Published: 2 May 2012
Ceruminous adenoma of the external auditory canal (EAC) is a rare type of tumour that is diagnosed histologically. However, the clinical behaviour of these tumours remains controversial. Here, we report a case of ceruminous adenoma of the EAC and expression of a hypoxia marker.
A 78-year-old man presented with a 6-month history of recurrent otorrhoea in the right ear. Surgery was performed by the transmeatal approach with total removal of the mass. Histopathology revealed a ceruminous adenoma. Tumour cells were positive for CK, S-100 protein, Glut-1, HIF-1α, PI3K and p-Akt. There was no evidence of recurrence at last follow-up 27 months after the operation.
Ceruminous adenoma of the EAC is a rare tumour. The treatment of choice is wide local excision with clear margins. To our knowledge, this is the first report of Glut-1 expression and the PI3K/Akt pathway in ceruminous adenoma of the EAC.
KeywordsCeruminous adenoma External auditory canal Glucose transporter 1
Ceruminous adenoma of the external auditory canal (EAC) is a rare type of tumour. According to the classification of Mills et al. , this is a benign lesion and only approximately 150 cases of ceruminous adenoma at this level have been reported worldwide to date ; consequently, surgeons and pathologists have little experience with these neoplasms. Due to the limited number of cases, there has been considerable confusion regarding the diagnosis, nomenclature, and behaviour of ceruminous gland neoplasms . Most cases follow a benign clinical course, and surgical resection with margins free of neoplasm is typically curative [1, 2]. Some authors have suggested that histological examination of benign tumours of the EAC cannot predict biological behaviour and that all tumours should therefore be treated as potentially malignant . Immunohistochemical staining for proteins such as CK5/6 and S-100 is an auxiliary method that has contributed to the differential diagnosis of ceruminous gland tumours [1, 5].
The growth of tumours is highly dependent on glucose as the major energy source. Overexpression of glucose transporter 1 (Glut-1) has been demonstrated in many types of human tumour, including some benign tumours, such as benign salivary gland tumours [6, 7], haemangiomas  and nerve sheath tumours . This has also been interpreted as an adaptation to intermittent hypoxia, which occurs as a tumour outgrows its blood supply. In addition to its role as a glucose transporter, Glut-1 is known to play an important role in the cellular response to hypoxia, as a downstream target of hypoxia-inducible factor-1α (HIF-1α). The HIF complex then binds to hypoxia-responsive elements (HRE) in target genes and activates their transcription. In addition to proline hydroxylation, other regulatory pathways, including the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, have been implicated in the control of HIF-1α protein expression and Glut-1 expression [10, 11].
Here, we present a case of ceruminous adenoma in the EAC expressing Glut-1, HIF-1α, PI3K and p-Akt, which to our knowledge has not been reported previously in ceruminous gland tumours.
At final follow-up, 27 months after the operation, there was no evidence of recurrence.
Discussion and Conclusions
Ceruminous gland tumours are uncommon lesions arising from the EAC, the behaviour of which is still unclear. In contrast to the view that most ceruminous gland tumours can be classified accurately histologically as either benign or malignant , some authors have suggested that all ceruminous gland tumours should be regarded as potentially malignant [4, 12]. Complete surgical resection is the treatment of choice and is associated with long-term tumour-free survival. Recurrence on follow-up may occur . However, there has been no recurrence in our case after 27 months of follow-up.
The differential diagnosis of ceruminous adenoma includes ceruminous adenocarcinoma, neuroendocrine adenoma of the middle ear, parotid pleomorphic adenoma, meningioma and paraganglioma . The results of immunohistochemical analyses are identical to those of normal cerumen glands, supporting the histogenesis of this benign neoplasm [1, 5]. All adenomas have a dual cell population, composed of inner luminal epithelial cells subtended by basal myoepithelial cells adjacent to the basement membrane. These cell populations are accentuated in immunohistochemical studies; the luminal cells are strongly immunoreactive for cytokeratin 7, whereas the basal myoepithelial cells are strongly and diffusely reactive for S-100 protein [1, 5].
In the present case, the neoplastic cells were richer, showed active growth, and were positive for CK and S-100 protein, suggesting possible roles of these proteins in the development of these cells. Glut-1 overexpression in tumour tissue is involved in the cellular response to hypoxia, as a downstream target of HIF-1α [8, 13]. Glut-1 upregulation and subsequent overexpression of Glut-1 receptors on the plasma membrane of various tumour cells are thought to allow escape from the apoptosis-inducing effects of hypoxic environments . HIF-1α modulates the expression of many genes involved in various processes, such as angiogenesis and pH regulation, and Glut-1 expression has a central role in this process. PI3K/AKT signalling plays an important role in the adaptive response of tumour cells to hypoxia [14, 15]. To some extent, under the control of AKT, information on AKT activation status could substantially add to the predictive potential of endogenous tumour markers (Glut-1, HIF-1α) [10, 11, 14]. Glut-1, HIF-1α, PI3K and p-Akt expression in neoplastic cells may be important in the development of a ceruminous adenoma.
In conclusion, ceruminous adenoma of the EAC is rare type of tumour that is diagnosed histologically. The treatment of choice is wide local excision with clear margins. Glut-1, HIF-1α, PI3K and p-Akt expression in neoplastic cells may be important for the development of ceruminous adenoma.
Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
External auditory canal
Hypoxia-inducible factor 1-α
Phosphatidylinositol 3-kinase /Akt pathway
Alpha-smooth muscle actin.
The immunohistochemical analysis for this studies was supported by Health Bureaue of Zhejiang Provincial (contract grant number: 2009B042 and 2010KYA062)
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