Open Access

Meta-analysis of the impact of human papillomavirus (HPV) on cancer risk and overall survival in head and neck squamous cell carcinomas (HNSCC)

  • Farshid Dayyani1, 2Email author,
  • Carol J Etzel3,
  • Mei Liu3,
  • Chung-Han Ho3,
  • Scott M Lippman1 and
  • Anne S Tsao1
Head & Neck Oncology20102:15

DOI: 10.1186/1758-3284-2-15

Received: 15 June 2010

Accepted: 29 June 2010

Published: 29 June 2010

Abstract

Background

HPV is important in a subset of HNSCC. Our meta-analysis determined the clinical characteristics of HPV-related HNSCC.

Method

Pubmed search terms "HPV" and "HNSCC" were used to identify 34 studies since 1980. We obtained pooled adjusted odds ratio (OR) or hazard ratio (HR) using random or fixed-effects model and compared OS depicted in forest plot.

Results

A total of 5681 patients were included. The prevalence of HPV+ tumors was 22%, with 86.7% of HPV16+ genotype. The OR for HNSCC in HPV16+ patients was 4.44 (95% CI = 2.87-6.02). HPV status was associated with p16 expression (adj OR = 3.00; 0.90-9.70), and HPV+ tumors were less likely to harbor p53 mutations (adj OR = 0.21; 0.04-0.38). The HR for death in HPV+ patients was 0.42 (0.27-0.57). HPV+ HNSCC also had a better response to therapy.

Conclusion

HPV+ HNSCC are established as a separate biologic entity. Prospective trials are needed to establish the optimal therapy for HPV+ HNSCC.

Introduction

Squamous cell carcinoma of the head and neck (HNSCC) has an estimated incidence of 35,310 new cases in 2008 in the United States, with an expected 7590 deaths due to these cancers. The male to female ratio is approximately 2:1[1]. A recent analysis of the Surveillance, Epidemiology and End Results (SEER) database showed in younger U.S. populations (ages 20-44 years) that while there has been an increase in incidence of tonsillar squamous cell carcinomas from 1973 to 2001, the incidence of squamous cell carcinomas in all other oral and pharyngeal sites remained constant or decreased[2]. A similar rise in the incidence of tonsillar squamous cell carcinomas from 1970-2002 has been shown in Sweden and has been associated with presence of human papillomavirus (HPV)[3]. For more than 30 years, certain genotypes of "high-risk" HPVs have been known to be involved in the pathogenesis of cervical cancers[4]. Recent studies have implicated high-risk HPV as a risk factor for HNSCC, independent of the traditional risk factors, which include tobacco abuse and ethanol consumption[57]. While high-risk HPV related HNSCC appear to be associated with certain sexual behaviors, such as oral sex and increasing numbers of sexual partners, there is a lack of association with smoking and drinking[5, 8]. Clinically, high-risk-HPV related HNSCC tend to present with lymph node positive disease and originate from the oropharynx, while histologically these tumors are usually high-grade and can be described as exhibiting a basaloid morphology [6, 9, 10]. On a molecular level, the HPV oncoproteins E6 and E7 are implied in tumorigenesis and are known to induce degradation of the tumor suppressors p53 and pRB, respectively[11, 12]. Another hallmark of HPV related HNSCC is lack of p53 mutations[13] and p16 protein overexpression[1416], a result of loss of transcriptional repression which occurs as a response to upstream signals during early tumorigenesis[17]. Studies suggest that p16 immunohistochemical expression can function as a surrogate marker to predict treatment outcome in HNSCC[14, 18].

Recent emerging data indicates that HPV related HNSCC define a unique population of patients with distinct biology that likely should be treated separately from non-HPV related HNSCC[19]. The aims of this meta-analysis are to consolidate the available data, improve understanding of the biology of HPV related HNSCC, and thereby identify goals for future research and trial design in HNSCC.

Methods

Article selection

To identify articles, we searched the Pubmed database, combining permutations of the terms ["human papillomavirus" or "HPV] and ["squamous cell carcinoma" or "cancer"] and ["oropharyngeal" or "oropharynx" or "head and neck" or "tonsil"]. Additional articles from reference lists of retrieved articles were reviewed and included as deemed appropriate. Only articles which solely or separately reported on oropharyngeal cancers, were published from 1980-2008 in English language, and reported HPV prevalence were considered for inclusion. A total of 34 articles met our inclusion criteria for this meta-analysis.

Statistical Analyses

The presence of study heterogeneity was evaluated using the Chi-squared based Q test. In order to quantify the extent (estimated as a percent ranging from 0% to 100%) of any existing heterogeneity in the meta-analyses, the I2 index was used. Pooled adjusted odds ratios (ORs) for risk or hazard ratios (HRs) for overall survival were obtained using a fixed-effects model (or random-effects model if needed due to among-study heterogeneity). If adjusted ORs or HRs were not available, then we used the same methods as above to pool unadjusted point estimates of risk and survival. Potential sources of publication bias were detected using a funnel plot which is simple scatter plots of the effect of interest versus study size. An asymmetrical funnel plot is indicative of potential publication bias. If necessary, we omitted studies prior to the final meta-analytic estimate that were identified as influential (those outside the allowable bounds based on the presumption of absence of both heterogeneity and publication bias) in the funnel plot. A forest plot was constructed to visually compare all included studies with the final pooled statistic. When possible, we also performed the above meta-analyses for the subset of oropharyngeal cancers. All analyses were completed using STATA 10.0.

Results

HPV Prevalence

A total of 5681 patients in 34 studies were included. To detect HPV DNA in tumor biopsies, 33 studies used PCR, and one study used fluorescence in situ hybridization (FISH). We established a prevalence of HPV among all head and neck squamous cell carcinoma (HNSCC) patients of 21.95% (95% CI = 0.21-0.23). The most prevalent HPV genotype was HPV-16, representing 86.69% (95% CI = 0.85-0.89) of all HPV positive tumors (Table 1 and 2). The other two most commonly detected genotypes were HPV-18 and -33 (data not shown). In the publications which included information on the subgroup of oropharyngeal cancers, the prevalence of HPV was 41% (379 of 925 patients; 95% CI = 0.38-0.44).
Table 1

HPV Prevalence in 34 trials (N = 5681)

Title

Patients (n)

No. of HPV positive (n)

Prevalence HPV %

No. of HPV16 (n)

Prevalence HPV16 in HPV (%)

Mork rtl, et al. 2001

292

88

30.14%

35

39.77%

D'Souza, et al. 2007

100

40

40.00%

35

87.50%

Gillison, et al. 2000

253

62

24.51%

56

90.32%

Herrero, et al. 2003

1670

56

3.35%

21

37.50%

Smith, et al. 2004

201

46

22.89%

39

84.78%

Braakhuis, et al. 2004

143

24

16.78%

24

100.00%

Fakhry, et al. 2008

96

38

39.58%

36

94.74%

Teman, et al. 2008

105

12

11.43%

0

0.00%

Weinberger, et al. 2006

77

47

61.04%

47

100.00%

Licitra, et al. 2006

90

17

18.89%

17

100.00%

Worden, et al. 2008

66

27

40.91%

27

100.00%

Shindoh, et al. 1995

77

24

31.17%

24

100.00%

Lindel, et al. 2001

99

14

14.14%

11

78.57%

Klussmann, et al. 2001

98

25

25.51%

21

84.00%

Perrone, et al. 2007

77

16

20.78%

16

100.00%

Kutler, et al. 2003

25

21

84.00%

19

90.48%

Smith, et al. 2004

193

38

19.69%

33

86.84%

Baez, et al. 2004

118

52

44.07%

52

100.00%

Dahlgren, et al. 2004

110

12

10.91%

9

75.00%

Hoffmann, et al. 2004

73

28

38.36%

28

100.00%

Slebos, et al. 2006

36

8

22.22%

8

100.00%

Hammarstedt, et al. 2006

203

99

48.77%

86

86.87%

Ragin, et al. 2006

125

30

24.00%

30

100.00%

Reimers, et al. 2007

106

30

28.30%

29

96.67%

Furniss, et al. 2007

486

145

29.84%

145

100.00%

Smith, et al. 2008

301

81

26.91%

77

95.06%

Pintos, et al. 2008

72

14

19.44%

13

92.86%

Charfi, et al. 2008

52

32

61.54%

27

84.38%

Westra, et al. 2008

89

12

13.48%

12

100.00%

Hafkamp, et al. 2008

81

33

40.74%

33

100.00%

Friesland, et al. 2001

34

14

41.18%

14

100.00%

De Petrini, et al. 2006

47

20

42.55%

20

100.00%

Wittekindt, et al. 2005

34

18

52.94%

16

88.89%

Strome, et al. 2002

52

24

46.15%

21

87.50%

 

5681

1247

21.95%

1081

86.69%

Table 2

Selected characteristics of the 34 studies

  

N

%

Confounding factors

Age

22

64.71%

 

Gender

24

70.59%

 

Smoking

15

44.12%

 

Alcohol

15

44.12%

Study type

Case-control

6

17.65%

 

Nested Case Control

1

2.94%

 

Multi-Center Case Control

1

2.94%

 

Case Series

3

8.82%

 

Retrospective

20

58.82%

 

Prospective

3

8.82%

Sample size

< 50

5

14.71%

(No. of patients)

50-99

14

41.18%

 

100-149

7

20.59%

 

>150

8

23.53%

Prevalence HPV

< 25%

14

41.18%

 

25%-50%

14

41.18%

 

50%-75%

5

14.71%

 

>75%

1

2.94%

Prevalence HPV-16

< 25%

16

47.06%

 

25%-50%

13

38.24%

 

50%-75%

4

11.76%

 

>75%

1

2.94%

Method of Detection

PCR

33

97.06%

 

FISH

1

2.94%

Country

USA

14

41.18%

 

Canada

1

2.94%

 

Puerto Rico

1

2.94%

 

France

2

5.88%

 

Germany

4

11.76%

 

Netherlands

2

5.88%

 

Italy

3

8.82%

 

Switzerland

1

2.94%

 

Norway, Finland, Sweden

1

2.94%

 

Sweden

3

8.82%

 

Japan

1

2.94%

 

International

1

2.94%

HPV as Risk Factor for HNSCC

Overall, positive HPV status (any high-risk genotype) conferred an increased risk for HNSCC (adjusted OR = 1.83; 95% CI = 1.04-2.62; p < 0.0001) as seen in Figure 1A. Risk for HNSCC among HPV-16 positive patients was 4.44 times (95% CI = 2.87-6.02; p < 0.0001) that of HPV-16 negative patients (Figure 1B). As a validation control for our meta-analysis we assessed the OR of two known risk factors for HNSCC, tobacco and alcohol consumption[20, 21]. We confirmed that heavy smokers and alcohol drinkers are at increased risk for HNSCC (smoking OR = 3.53; 95% CI = 2.69-4.64, p < 0.0001, and alcohol consumption OR = 4.04; 95% CI = 3.20-5.09, p < 0.0001). Table 3 shows the association of HPV status with prognostic factors for HNSCC (age, gender, and tumor stage) and known risk factors for HNSCC (ethanol and tobacco consumption)
https://static-content.springer.com/image/art%3A10.1186%2F1758-3284-2-15/MediaObjects/13051_2010_Article_108_Fig1_HTML.jpg
Figure 1

Meta-analyses for adjusted odds ratio (OR) for HPV as risk factor for HNSCC Risk for having HNSCC is increased with all types of high-risk HPV reported (A) and risk is even more pronounced in cases with HPV-16 infection versus HPV negative controls (B).

Table 3

Association of HPV status with gender, age, tumor stage, alcohol and tobacco consumption

Demographic

# Studies

HPV status

Smoking status

N

%

Tobacco

17

HPV+

Ever

1068

55.9

   

Never

948

44.1

  

HPV-

Ever

2179

74.5

   

Never

677

25.5

Ethanol

16

HPV+

Ever

1548

66.8

   

Never

768

33.2

  

HPV-

Ever

2186

75.4

   

Never

713

24.6

Stage

10*

HPV+

I-II

78

26.7

   

III-IV

214

73.3

  

HPV-

I-II

259

42.8

   

III-IV

346

57.2

LN Stage

6

HPV+

N0

45

33.5

   

N1-N3

123

66.5

  

HPV-

N0

204

45.9

   

N1-N3

240

54.1

Gender

18

HPV+

M

1525

67.8

   

F

724

32.2

  

HPV-

M

2010

66.1

   

F

1033

33.9

Median Age

5**

HPV+

55 years

  
  

HPV-

60 years

  

HPV: human papillomavirus; M: male; F: female; LN, lymph node; * 2 studies compared stage I-III vs. stage IV; ** 5 studies reported the median age of the subjects.

HPV and Survival

Survival was improved in HPV positive patients compared to HPV negative patients (HR = 0.42; 95% CI = 0.27-0.56, p < 0.0001) (Figure 2A). The survival benefit was similar in HPV-16 positive patients (adjusted HR = 0.41; 95% CI = 0.21-0.61, p < 0.0001). The improved survival in HPV positive patients was even more pronounced in the subgroup of oropharyngeal cancers (adjusted HR = 0.40; CI = 0.18-0.61, p < 0.0001) (Figure 2B).
https://static-content.springer.com/image/art%3A10.1186%2F1758-3284-2-15/MediaObjects/13051_2010_Article_108_Fig2_HTML.jpg
Figure 2

Overall survival of HPV positive patients compared to HPV negative patients in unselected HNSCC patients (A) and in the subgroup of patients with oropharyngeal cancers (B).

HPV and Treatment Response

A total of ten publications included treatment information, but only two reported statistical information on treatment response to radiotherapy and two reported efficacy results to chemotherapy/radiotherapy (Table 4). HPV positive HNSCC have a better response to radiotherapy compared to HPV negative HNSCC (non-adjusted OR = 4.07; 95% CI = 1.48-11.18, p = 0.006). Similarly, patients who are positive for HPV have an improved response to concurrent chemo-radiation (non-adjusted OR = 2.87; 95% CI = 1.29-6.41, p = 0.01).
Table 4

Treatment type and country of origin of studies that reported treatment information

Author, Year

Type of Treatment

Country

Fakhry, et al. 2008

CTX followed by Chemo-XRT

United States

Weinberger, et al. 2006

XRT ± surgery

United States

Worden, et al. 2008

CTX followed by Chemo-XRT or XRT/surgery

United States

Lindel, et al. 2001

XRT

Switzerland

Dahlgren, et al. 2004

XRT

Sweden

Smith, et al. 2008

XRT ± surgery

United States

Hafkamp, et al. 2008

CTX ± XRT ± surgery

Netherlands

Friesland, et al. 2001

XRT

Sweden

CTX: Chemotherapy; XRT: Radiotherapy; Chemo-XRT: concurrent chemo-radiation

Correlation with p16 and p53

Several studies reported p16 immunohistochemical expression in HPV positive HNSCC[2229]. We found that HPV status was associated with p16 expression (adjusted OR = 3.00; 95% CI = 0.90-9.70, p = 0.18), and HPV positive tumors were less likely to harbor p53 mutations (adjusted OR = 0.21; 95% CI = 0.04-0.38, p = 0.015) (Table 5). Confirming the notion that p16 expression might be used as a surrogate for HPV status in HNSCC, we show that p16 positive HNSCC have reduced hazard ratio for death (adjusted HR = 0.22; 95% CI = 0.02-0.41, p = 0.028).
Table 5

Meta-analyses of association between p53 mutations, p16 and HPV

  

Before outlier exclusion

After outlier exclusion

Outcome

Marker

No. of Studies

Adjusted OR (95% CI)

No. of Studies

Adjusted OR (95% CI)

HPV positive

p53 (mutated)

4

0.21(0.04-0.38)

4

0.21(0.04-0.38)

     

P = 0.015

HPV positive

p16

2

5.02(1.08-8.97)

1

3.00(0.90-9.70)

 

IHC

   

p = 0.181

Discussion

We have conducted a literature review and large meta-analysis in HPV related HNSCC to determine the prevalence of the disease and assess the ramifications on survival and associated molecular biomarkers. Given prior published reports of a higher prevalence of HPV in carcinomas of the oropharynx, we concentrated this study on oropharyngeal carcinomas to enrich and focus the meta-analysis. Therefore, the number of studies included in our review is smaller than other previously reported meta-analyses with different inclusion criteria[30, 31]. Several other meta-analyses on HPV-related HNSCC have been published before[3032], but to our knowledge, none of the prior reports have described within the same report, the impact of HPV on survival, response to therapy, as well as the association with molecular markers such as p16. In addition, our report includes recently published prospective studies that were not available at the time of publication of previous meta-analyses. Our data reports the prevalence of HPV-related HNSCC as 21.95% and is in accordance with other recently published reports which estimate the prevalence of HPV at 20-26% [25, 3032]. As expected, we also confirmed a higher prevalence of HPV-related tumors in the oropharynx (40.97%) in agreement with prior studies[31, 33]. Virtually all of the studies included used PCR to detect HPV DNA, a method with slightly higher sensitivity than in situ hybridization[31]. The higher sensitivity of the detection method used suggests that the herein reported prevalence of HPV associated HNSCC is more likely to reflect the actual prevalence of HPV as compared to studies which use less sensitive methods, although given the paucity of data regarding the exact specificity of PCR in this setting does not enable a precise account of the number of false-positive patients included in these studies. The relatively wide range of the prevalence of HPV in HNSCC in the published literature confirms that in addition to geographical differences, the method of HPV detection is critical for accurate assessment of the HPV status.

In the current study we confirmed the role of p16 immunohistochemical expression as a surrogate marker for HPV infection in HNSCC, which is not only positively associated with HPV infection but also predicts for an improved survival outcome[14, 28, 29]. The HPV oncoprotein E7 is known to induce degradation of pRb, which in turn leads to upregulation of p16[34]. Therefore, overexpression of p16 is considered to be one of the molecular hallmarks of HPV positive HNSCC[4, 23, 35, 36] and has recently been shown to identify HPV related oropharyngeal cancers with favorable outcomes[16]. In a recent prospective trial, an analysis of tumor tissue (n = 323) collected from RTOG 0129 using HPV in situ hybridization (ISH) and p16 immunohistochemistry (IHC) demonstrated that p16 correlated well with HPV ISH with 96% of HPV positive tumors also expressing p16[19]. An additional 19% (n = 22) were p16 positive but HPV negative. Both HPV and p16 positive status predicted for an improved survival. This finding of p16 IHC positive tumors identifying more patients than HPV ISH was also seen in an additional study called HeadStart[37]. This trial compared HPV ISH and p16 IHC on tumor specimens from two arms of therapy, cisplatin-radiation versus cisplatin-tirapazamine-radiation, and suggested that p16 IHC may identify a higher percentage of HPV positive tumors than the HPV ISH assay. Given the debate regarding the best detection method for HPV[38], our data from the meta-analysis confirms that p16 IHC could be used in future in prospective trials as a reliable surrogate marker for HPV related HNSCC[19, 37, 39]. As the above two recent studies (RTOG and HeadStart) have only been reported in abstract form, we did not include them in our meta-analysis.

As HPV-related oropharyngeal cancers are reported to be rising in incidence[2, 3], it is important to consider treating this disease as a unique entity. Our data in congruence with other studies indicate that HPV positive HNSCC have a distinct biology, and are more responsive to treatment with radiotherapy and chemotherapy. In addition to treatment response, we and others have shown an improved survival in patients with HPV positive HNSCC, with a 60% lower risk of death. Our survival results are slightly different from other reports; a recent meta-analysis reported a death hazard ratio of 0.85. This discrepancy can be explained by the addition of the recent prospective trials[10, 14, 40, 41] in our analysis which were not included in prior reports. Our meta-analysis also confirms a better response to treatment in HPV positive HNSCC in accordance with recently published trials[10, 14, 39, 41, 42]. Recent reports have highlighted the importance of the negative influence of smoking on prognosis within HPV positive HNSCC[19, 43], and future studies are required to examine this relationship in a prospective manner.

There are several limitations inherent to our methodology, which was based on literature review, not individual patient data. While the total number of patients included in our meta-analysis is large, for many specific associations the number of the studies that actually could be combined to provide information is relatively small. This is a limitation which unfortunately cannot be overcome without access to primary data from all included publications. Also, there is overlap of the same patient series included in multiple reports. We tried to account for this fact as much as possible by reviewing the author list and institution of each paper and including only the largest study if several were apparently from the same group. Finally, given the heterogeneity of the kinds of studies our meta-analysis was based on, it is difficult to combine conclusions drawn, e.g. assessing HPV as risk factor for HNSCC from case series, retrospective and prospective trials. There is no valid formulation to give adequate weight to the results of each kind of the studies mentioned. In spite of the limitations inherent to this type of a meta-analysis, our results are in keeping with published literature and our current understanding of the natural biology of HPV-related HNSCC. We also performed as a validation control a meta-analysis for the established HNSCC risk factors smoking and alcohol consumption. Our results confirmed prior published results [20, 21].

For the future, there is a need for randomized prospective data to identify the optimal treatment for patients based on the tumor's HPV status and to also develop potential prevention programs. First, given the apparent improved response to current treatment modalities, patients with HPV-related HNSCC may be able to receive adequate curative intent therapy from a reduction in radiation or chemotherapy dose. These issues will require future clinical trial designs to either prospectively stratify patients by HPV status or to conduct trials which are dedicated to HPV positive HNSCC. In addition, it is necessary to determine the best method of detecting the HPV status and validate whether p16 overexpression should be utilized as a surrogate or in conjunction with direct HPV testing. Given the etiologic involvement of the HPV oncoproteins E6 and E7 during carcinogenesis of these tumors[44], it is also conceivable that intervention with anti-viral drugs may enhance sensitivity of these tumors to cytotoxic drugs, with the goal of reducing the required dose of chemotherapeutic agents and the associated side effects. Exploratory biomarker studies will be needed to identify prognostic and predictive markers in order to tailor specific therapies (such as biologic agents) to the biology of the patients' malignancy, resulting in better tolerability of treatment with improved outcomes.

The issue of preventing HPV associated HNSCC from occurring requires investigation as well. Two recently approved vaccines to prevent HPV-related cervical cancers[45, 46] cover the two serotypes that are most prevalent in HPV-related HNSCC (HPV-16 and 18). In the context of the findings of our study, it is conceivable that extending vaccination to both sexes would prevent a significant number of future oropharyngeal cancers in both men and women. Also, the hypothesized benefits of HPV-vaccination would potentially include a reduced incidence of other HPV-related cancers (i.e. vulvar, vaginal, anal, and penile cancers)[47]. On the other hand, a recent study showed that vaccination of males on a population basis might not be cost-effective[48]. Even in women, controversy exists regarding the maximum age at which the HPV-vaccine should be given, and a recent simulation model suggested that vaccination beyond 30 years of age might also not be cost-effective[49]. Given all these uncertainties, it will therefore be of great importance to assess in prospective trials that include both sexes the efficacy of these vaccines to prevent HPV-related HNSCC.

Conclusions

HPV-related HNSCC comprise about 25% of all HNSCC. They are predominantly tumors of the oropharynx, and exhibit a separate biologic behavior including improved response to (chemo)-radiation and survival compared to HPV-negative HNSCC. The optimal therapy for this subset of patients remains unclear, but the growing data supports the theory that this subset of patients should be treated differently than HPV negative patients. In addition to this avenue of clinical research, identifying peripheral surrogate biomarkers and possible screening and prevention methods is an important field of future investigation.

Declarations

Authors’ Affiliations

(1)
Department of Thoracic/Head & Neck Medical Oncology, University of Texas MD Anderson Cancer Center
(2)
Division of Cancer Medicine, University of Texas MD Anderson Cancer Center
(3)
Department of Epidemiology, University of Texas MD Anderson Cancer Center

References

  1. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ: Cancer statistics, 2008. CA: a cancer journal for clinicians. 2008, 58 (2): 71-96. 10.3322/CA.2007.0010.Google Scholar
  2. Shiboski CH, Schmidt BL, Jordan RC: Tongue and tonsil carcinoma: increasing trends in the U.S. population ages 20-44 years. Cancer. 2005, 103 (9): 1843-1849. 10.1002/cncr.20998.View ArticlePubMedGoogle Scholar
  3. Hammarstedt L, Lindquist D, Dahlstrand H, Romanitan M, Dahlgren LO, Joneberg J, Creson N, Lindholm J, Ye W, Dalianis T, et al: Human papillomavirus as a risk factor for the increase in incidence of tonsillar cancer. International journal of cancer. 2006, 119 (11): 2620-2623. 10.1002/ijc.22177.View ArticleGoogle Scholar
  4. zur Hausen H: Papillomaviruses and cancer: from basic studies to clinical application. Nature reviews. 2002, 2 (5): 342-350. 10.1038/nrc798.PubMedGoogle Scholar
  5. D'Souza G, Kreimer AR, Viscidi R, Pawlita M, Fakhry C, Koch WM, Westra WH, Gillison ML: Case-control study of human papillomavirus and oropharyngeal cancer. The New England journal of medicine. 2007, 356 (19): 1944-1956. 10.1056/NEJMoa065497.View ArticlePubMedGoogle Scholar
  6. Gillison ML, Koch WM, Capone RB, Spafford M, Westra WH, Wu L, Zahurak ML, Daniel RW, Viglione M, Symer DE, et al: Evidence for a causal association between human papillomavirus and a subset of head and neck cancers. Journal of the National Cancer Institute. 2000, 92 (9): 709-720. 10.1093/jnci/92.9.709.View ArticlePubMedGoogle Scholar
  7. Gillison ML, Shah KV: Human papillomavirus-associated head and neck squamous cell carcinoma: mounting evidence for an etiologic role for human papillomavirus in a subset of head and neck cancers. Current opinion in oncology. 2001, 13 (3): 183-188. 10.1097/00001622-200105000-00009.View ArticlePubMedGoogle Scholar
  8. Gillison ML, D'Souza G, Westra W, Sugar E, Xiao W, Begum S, Viscidi R: Distinct risk factor profiles for human papillomavirus type 16-positive and human papillomavirus type 16-negative head and neck cancers. Journal of the National Cancer Institute. 2008, 100 (6): 407-420. 10.1093/jnci/djn025.View ArticlePubMedGoogle Scholar
  9. Andl T, Kahn T, Pfuhl A, Nicola T, Erber R, Conradt C, Klein W, Helbig M, Dietz A, Weidauer H, et al: Etiological involvement of oncogenic human papillomavirus in tonsillar squamous cell carcinomas lacking retinoblastoma cell cycle control. Cancer research. 1998, 58 (1): 5-13.PubMedGoogle Scholar
  10. Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, Forastiere A, Gillison ML: Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. Journal of the National Cancer Institute. 2008, 100 (4): 261-269. 10.1093/jnci/djn011.View ArticlePubMedGoogle Scholar
  11. Dyson N, Howley PM, Munger K, Harlow E: The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science (New York, NY). 1989, 243 (4893): 934-937.View ArticleGoogle Scholar
  12. Werness BA, Levine AJ, Howley PM: Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science (New York, NY). 1990, 248 (4951): 76-79.View ArticleGoogle Scholar
  13. Westra WH, Taube JM, Poeta ML, Begum S, Sidransky D, Koch WM: Inverse relationship between human papillomavirus-16 infection and disruptive p53 gene mutations in squamous cell carcinoma of the head and neck. Clin Cancer Res. 2008, 14 (2): 366-369. 10.1158/1078-0432.CCR-07-1402.View ArticlePubMedGoogle Scholar
  14. Kumar B, Cordell KG, Lee JS, Worden FP, Prince ME, Tran HH, Wolf GT, Urba SG, Chepeha DB, Teknos TN, et al: EGFR, p16, HPV Titer, Bcl-xL and p53, sex, and smoking as indicators of response to therapy and survival in oropharyngeal cancer. J Clin Oncol. 2008, 26 (19): 3128-3137. 10.1200/JCO.2007.12.7662.PubMed CentralView ArticlePubMedGoogle Scholar
  15. O'Regan EM, Toner ME, Finn SP, Fan CY, Ring M, Hagmar B, Timon C, Smyth P, Cahill S, Flavin R, et al: p16(INK4A) genetic and epigenetic profiles differ in relation to age and site in head and neck squamous cell carcinomas. Human pathology. 2008, 39 (3): 452-458. 10.1016/j.humpath.2007.08.004.View ArticlePubMedGoogle Scholar
  16. Weinberger PM, Yu Z, Haffty BG, Kowalski D, Harigopal M, Brandsma J, Sasaki C, Joe J, Camp RL, Rimm DL, et al: Molecular classification identifies a subset of human papillomavirus--associated oropharyngeal cancers with favorable prognosis. J Clin Oncol. 2006, 24 (5): 736-747. 10.1200/JCO.2004.00.3335.View ArticlePubMedGoogle Scholar
  17. Mroz EA, Baird AH, Michaud WA, Rocco JW: COOH-terminal binding protein regulates expression of the p16INK4A tumor suppressor and senescence in primary human cells. Cancer research. 2008, 68 (15): 6049-6053. 10.1158/0008-5472.CAN-08-1279.View ArticlePubMedGoogle Scholar
  18. Nichols AC, Faquin WC, Westra WH, Mroz EA, Begum S, Clark JR, Rocco JW: HPV-16 infection predicts treatment outcome in oropharyngeal squamous cell carcinoma. Otolaryngol Head Neck Surg. 2009, 140 (2): 228-234. 10.1016/j.otohns.2008.11.025.View ArticlePubMedGoogle Scholar
  19. Gillison HJ, Westra W, Chung C, Jordan R, Rosenthal D, Nguyen-Tan P, Spanos WJ, Redmond KP, Ang K: Survival outcomes by tumor human papillomavirus (HPV) status in stage III-IV oropharyngeal cancer (OPC) in RTOG 0129. J Clin Oncol. 2009, 27: 15s-10.1200/JCO.2008.21.7695.View ArticleGoogle Scholar
  20. Murata M, Takayama K, Choi BC, Pak AW: A nested case-control study on alcohol drinking, tobacco smoking, and cancer. Cancer detection and prevention. 1996, 20 (6): 557-565.PubMedGoogle Scholar
  21. Spitz MR: Epidemiology and risk factors for head and neck cancer. Seminars in oncology. 1994, 21 (3): 281-288.PubMedGoogle Scholar
  22. Charfi L, Jouffroy T, de Cremoux P, Le Peltier N, Thioux M, Freneaux P, Point D, Girod A, Rodriguez J, Sastre-Garau X: Two types of squamous cell carcinoma of the palatine tonsil characterized by distinct etiology, molecular features and outcome. Cancer letters. 2008, 260 (1-2): 72-78. 10.1016/j.canlet.2007.10.028.View ArticlePubMedGoogle Scholar
  23. Hafkamp HC, Manni JJ, Haesevoets A, Voogd AC, Schepers M, Bot FJ, Hopman AH, Ramaekers FC, Speel EJ: Marked differences in survival rate between smokers and nonsmokers with HPV 16-associated tonsillar carcinomas. International journal of cancer. 2008, 122 (12): 2656-2664. 10.1002/ijc.23458.View ArticleGoogle Scholar
  24. Licitra L, Perrone F, Bossi P, Suardi S, Mariani L, Artusi R, Oggionni M, Rossini C, Cantu G, Squadrelli M, et al: High-risk human papillomavirus affects prognosis in patients with surgically treated oropharyngeal squamous cell carcinoma. J Clin Oncol. 2006, 24 (36): 5630-5636. 10.1200/JCO.2005.04.6136.View ArticlePubMedGoogle Scholar
  25. Ragin CC, Taioli E, Weissfeld JL, White JS, Rossie KM, Modugno F, Gollin SM: 11q13 amplification status and human papillomavirus in relation to p16 expression defines two distinct etiologies of head and neck tumours. British journal of cancer. 2006, 95 (10): 1432-1438. 10.1038/sj.bjc.6603394.PubMed CentralView ArticlePubMedGoogle Scholar
  26. Reimers N, Kasper HU, Weissenborn SJ, Stutzer H, Preuss SF, Hoffmann TK, Speel EJ, Dienes HP, Pfister HJ, Guntinas-Lichius O, et al: Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer. International journal of cancer. 2007, 120 (8): 1731-1738. 10.1002/ijc.22355.View ArticleGoogle Scholar
  27. Slebos RJ, Yi Y, Ely K, Carter J, Evjen A, Zhang X, Shyr Y, Murphy BM, Cmelak AJ, Burkey BB, et al: Gene expression differences associated with human papillomavirus status in head and neck squamous cell carcinoma. Clin Cancer Res. 2006, 12 (3 Pt 1): 701-709. 10.1158/1078-0432.CCR-05-2017.View ArticlePubMedGoogle Scholar
  28. Smith EM, Wang D, Kim Y, Rubenstein LM, Lee JH, Haugen TH, Turek LP: P16INK4a expression, human papillomavirus, and survival in head and neck cancer. Oral oncology. 2008, 44 (2): 133-142. 10.1016/j.oraloncology.2007.01.010.View ArticlePubMedGoogle Scholar
  29. Wittekindt C, Gultekin E, Weissenborn SJ, Dienes HP, Pfister HJ, Klussmann JP: Expression of p16 protein is associated with human papillomavirus status in tonsillar carcinomas and has implications on survival. Advances in oto-rhino-laryngology. 2005, 62: 72-80.PubMedGoogle Scholar
  30. Kreimer AR, Clifford GM, Boyle P, Franceschi S: Human papillomavirus types in head and neck squamous cell carcinomas worldwide: a systematic review. Cancer Epidemiol Biomarkers Prev. 2005, 14 (2): 467-475. 10.1158/1055-9965.EPI-04-0551.View ArticlePubMedGoogle Scholar
  31. Termine N, Panzarella V, Falaschini S, Russo A, Matranga D, Lo Muzio L, Campisi G: HPV in oral squamous cell carcinoma vs head and neck squamous cell carcinoma biopsies: a meta-analysis (1988-2007). Ann Oncol. 2008, 19 (10): 1681-1690. 10.1093/annonc/mdn372.View ArticlePubMedGoogle Scholar
  32. Ragin CC, Taioli E: Survival of squamous cell carcinoma of the head and neck in relation to human papillomavirus infection: review and meta-analysis. International journal of cancer. 2007, 121 (8): 1813-1820. 10.1002/ijc.22851.View ArticleGoogle Scholar
  33. Hobbs CG, Sterne JA, Bailey M, Heyderman RS, Birchall MA, Thomas SJ: Human papillomavirus and head and neck cancer: a systematic review and meta-analysis. Clin Otolaryngol. 2006, 31 (4): 259-266. 10.1111/j.1749-4486.2006.01246.x.View ArticlePubMedGoogle Scholar
  34. Li Y, Nichols MA, Shay JW, Xiong Y: Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Cancer research. 1994, 54 (23): 6078-6082.PubMedGoogle Scholar
  35. Klussmann JP, Gultekin E, Weissenborn SJ, Wieland U, Dries V, Dienes HP, Eckel HE, Pfister HJ, Fuchs PG: Expression of p16 protein identifies a distinct entity of tonsillar carcinomas associated with human papillomavirus. The American journal of pathology. 2003, 162 (3): 747-753.PubMed CentralView ArticlePubMedGoogle Scholar
  36. Li W, Thompson CH, Cossart YE, O'Brien CJ, McNeil EB, Scolyer RA, Rose BR: The expression of key cell cycle markers and presence of human papillomavirus in squamous cell carcinoma of the tonsil. Head & neck. 2004, 26 (1): 1-9. 10.1002/hed.10335.View ArticleGoogle Scholar
  37. Rischin DYR, Fisher R, Fox S, Le Q, Peters L, Choi J, O'Sullivan B, Giralt J, McArthur G: Prognostic significance of HPV and p16 status in patients with oropharyngeal cancer treated on a large international phase III trial. J Clin Oncol. 2009, 27: 15s-10.1200/JCO.2008.21.7695.View ArticleGoogle Scholar
  38. Campisi G, Giovannelli L: Controversies surrounding Human Papilloma Virus infection, head & neck vs oral cancer, implications for prophylaxis and treatment. Head & neck oncology. 2009, 1 (1): 8-10.1186/1758-3284-1-8.View ArticleGoogle Scholar
  39. Lassen P, Eriksen JG, Hamilton-Dutoit S, Tramm T, Alsner J, Overgaard J: Effect of HPV-Associated p16INK4A Expression on Response to Radiotherapy and Survival in Squamous Cell Carcinoma of the Head and Neck. J Clin Oncol. 2009, 27 (12): 1992-8. 10.1200/JCO.2008.20.2853.View ArticlePubMedGoogle Scholar
  40. Kumar B, Cordell KG, Lee JS, Prince ME, Tran HH, Wolf GT, Urba SG, Worden FP, Chepeha DB, Teknos TN, et al: Response to therapy and outcomes in oropharyngeal cancer are associated with biomarkers including human papillomavirus, epidermal growth factor receptor, gender, and smoking. International journal of radiation oncology, biology, physics. 2007, 69 (2 Suppl): S109-111.PubMed CentralView ArticlePubMedGoogle Scholar
  41. Worden FP, Kumar B, Lee JS, Wolf GT, Cordell KG, Taylor JM, Urba SG, Eisbruch A, Teknos TN, Chepeha DB, et al: Chemoselection as a strategy for organ preservation in advanced oropharynx cancer: response and survival positively associated with HPV16 copy number. J Clin Oncol. 2008, 26 (19): 3138-3146. 10.1200/JCO.2007.12.7597.PubMed CentralView ArticlePubMedGoogle Scholar
  42. Cohen MA, Basha SR, Reichenbach DK, Robertson E, Sewell DA: Increased viral load correlates with improved survival in HPV-16-associated tonsil carcinoma patients. Acta oto-laryngologica. 2008, 128 (5): 583-589. 10.1080/00016480701558880.View ArticlePubMedGoogle Scholar
  43. Worden HJ, Lee J, Eisbruch A, Wolf GT, Prince M, Moyer J, Teknos T, Chepeha DB, Bradford CR, Carey T: Association of tobacco (T) use with risk of distant metastases (DM), tumor recurrence, and death in patients (pts) with HPV-positive (+) squamous cell cancer of the oropharynx (SCCOP). J Clin Oncol. 2009, 27: 15s-10.1200/JCO.2008.21.7695.View ArticleGoogle Scholar
  44. Tran N, Rose BR, O'Brien CJ: Role of human papillomavirus in the etiology of head and neck cancer. Head & neck. 2007, 29 (1): 64-70. 10.1002/hed.20460.View ArticleGoogle Scholar
  45. Quadrivalent vaccine against human papillomavirus to prevent high-grade cervical lesions. The New England journal of medicine. 2007, 356 (19): 1915-1927. 10.1056/NEJMoa061741.
  46. Paavonen J, Jenkins D, Bosch FX, Naud P, Salmeron J, Wheeler CM, Chow SN, Apter DL, Kitchener HC, Castellsague X, et al: Efficacy of a prophylactic adjuvanted bivalent L1 virus-like-particle vaccine against infection with human papillomavirus types 16 and 18 in young women: an interim analysis of a phase III double-blind, randomised controlled trial. Lancet. 2007, 369 (9580): 2161-2170. 10.1016/S0140-6736(07)60946-5.View ArticlePubMedGoogle Scholar
  47. Chang Y, Brewer NT, Rinas AC, Schmitt K, Smith JS: Evaluating the impact of human papillomavirus vaccines. Vaccine. 2009, 27 (32): 4355-4362. 10.1016/j.vaccine.2009.03.008.View ArticlePubMedGoogle Scholar
  48. Kim JJ, Goldie SJ: Cost effectiveness analysis of including boys in a human papillomavirus vaccination programme in the United States. BMJ (Clinical research ed). 2009, 339: b3884-10.1136/bmj.b3884.View ArticleGoogle Scholar
  49. Kim JJ, Ortendahl J, Goldie SJ: Cost-effectiveness of human papillomavirus vaccination and cervical cancer screening in women older than 30 years in the United States. Annals of internal medicine. 2009, 151 (8): 538-545.PubMed CentralView ArticlePubMedGoogle Scholar

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